Teaching GuideTerm Faculty of Science |
Mestrado Universitario en Investigación Química e Química Industrial (Plan 2020) |
Subjects |
Fluorescence Spectroscopy and Photochemistry |
Contents |
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Identifying Data | 2023/24 | |||||||||||||
Subject | Fluorescence Spectroscopy and Photochemistry | Code | 610509108 | |||||||||||
Study programme |
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Descriptors | Cycle | Period | Year | Type | Credits | |||||||||
Official Master's Degree | 1st four-month period |
First | Optional | 3 | ||||||||||
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Topic | Sub-topic |
1. Fundamentals of electronic spectroscopy and fluorescence spectroscopy | Luminiscent phenomena. Radiative and nonradiative processes. Fluorescence excitation and emission spectra. Fluorescence quantum yield. Fluorescence lifetime. Effect of environment on fluorescence. |
2. Experimental techniques | Measurement of fluorescence spectra: the spectrofluorometer. Correction of excitation and emission spectra. Measurement of fluorescence lifetimes. Measurement of fluorescence polarization. Ultrafast techniques. Single-molecule fluorescence. Fluorescence Microscopy. |
3. Fluorescence quenching | Collisional or dynamic quenching. Stern-Volmer equation. Static quenching. Static and dynamic quenching. Applications to study complex formation and microheterogeneous systems. |
4. Excited electronic states and photochemistry | Excited-state complex formation: excimers and exciplexes. Photoinduced electron transfer. Photoinduced proton transfer. Other photochemical reactions. |
5. Electronic energy transfer | Electronic energy-transfer mechanisms. Förster Resonance Energy Transfer (FRET). Applications for the measurement of molecular distances and the study of supramolecular associations. Dexter mechanism of energy transfer: photosensitization and photodynamic therapy. |
6. Fluorescence probes | Classes of fluorescence probes: intrinsic and extrinsic. Green Fluorescence Protein. Quantum dots. Applications in biomedicine, analyses, environment, and materials studies. |
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