Teaching GuideTerm
Faculty of Science
  Home | galego | castellano | english | A A A |  
Mestrado Universitario en Investigación Química e Química Industrial (Plan 2020)
 Subjects
  Fluorescence Spectroscopy and Photochemistry
   Contents
Topic Sub-topic
1. Fundamentals of electronic spectroscopy and fluorescence spectroscopy Luminiscent phenomena. Radiative and nonradiative processes. Fluorescence excitation and emission spectra. Fluorescence quantum yield. Fluorescence lifetime. Effect of environment on fluorescence.
2. Experimental techniques Measurement of fluorescence spectra: the spectrofluorometer. Correction of excitation and emission spectra. Measurement of fluorescence lifetimes. Measurement of fluorescence polarization. Ultrafast techniques. Single-molecule fluorescence. Fluorescence Microscopy.
3. Fluorescence quenching Collisional or dynamic quenching. Stern-Volmer equation. Static quenching. Static and dynamic quenching. Applications to study complex formation and microheterogeneous systems.
4. Excited electronic states and photochemistry Excited-state complex formation: excimers and exciplexes. Photoinduced electron transfer. Photoinduced proton transfer. Other photochemical reactions.
5. Electronic energy transfer Electronic energy-transfer mechanisms. Förster Resonance Energy Transfer (FRET). Applications for the measurement of molecular distances and the study of supramolecular associations. Dexter mechanism of energy transfer: photosensitization and photodynamic therapy.
6. Fluorescence probes Classes of fluorescence probes: intrinsic and extrinsic. Green Fluorescence Protein. Quantum dots. Applications in biomedicine, analyses, environment, and materials studies.
Universidade da Coruña - Rúa Maestranza 9, 15001 A Coruña - Tel. +34 981 16 70 00  Soporte Guías Docentes