Teaching GuideTerm Faculty of Science |
Grao en Nanociencia e Nanotecnoloxía |
Subjects |
Fundamentals of Biotechnology |
Contents |
Identifying Data | 2023/24 | |||||||||||||
Subject | Fundamentals of Biotechnology | Code | 610G04029 | |||||||||||
Study programme |
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Descriptors | Cycle | Period | Year | Type | Credits | |||||||||
Graduate | 2nd four-month period |
Third | Obligatory | 6 | ||||||||||
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Topic | Sub-topic |
1. INTRODUCTION TO BIOTECHNOLOGY | Current concept of Biotechnology. History and development of Biotechnology. Outlook. Importance of Nanobiotechnology. |
2. CLONING | Purposes of molecular cloning. Basic steps of gene cloning. Polymerase chain reaction. DNA fragmentation: restriction enzymes. Binding of DNA molecules. Cloning and Nanotechnology. |
3. GENOMIC LIBRARIES | Library concept. Genomic DNA libraries. cDNA libraries. Expression libraries. Amplification, storage and replication of libraries. Libraries in Nanotechnology. |
4. PROTEIN ENGINEERING | Production of heterologous proteins in bacteria and yeast (Selection of microorganisms. Expression vectors. Expression in transformed cells. Secretion.) Production of recombinant proteins in animal cells (Expression vectors. Baculovirus-mediated protein expression in insect cell cultures ). Applications in Nanotechnology. |
5. PROTEIN INMOBILIZATION | Enzyme stability. Immobilized biocatalyst concept. immobilization systems. Nano-scale immobilization. |
6. TRANSFORMATION AND GENE EDITING | Concepts of modification, transformation and gene editing. Direct and indirect genetic transformation methods. Transgenic plants and animals. Transgenic foods. Nanotechnology in genetic modification. |
7. CELL AND TISSUE ENGINEERING | Introduction to animal and plant cell cultures. Types of crops. Cell culture requirements. Quantification of cellular parameters. contaminations. Cytotoxicity. |
8. ETHICAL AND LEGAL ASPECTS | Current situation of regulatory regulations, patents. Ethical issues, security, risks. Social perception. |
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