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Teaching GuideTerm Faculty of Science |
| Mestrado Universitario en Bioloxía Molecular , Celular e Xenética |
 Subjects |
| Protein Structure and Dynamics |
| Contents |
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| Identifying Data | 2019/20 | |||||||||||||
| Subject | Protein Structure and Dynamics | Code | 610441011 | |||||||||||
| Study programme |
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| Descriptors | Cycle | Period | Year | Type | Credits | |||||||||
| Official Master's Degree | 2nd four-month period |
First | Optional | 3 | ||||||||||
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| Topic | Sub-topic |
| Structural classification of proteins. | Structural domains of proteins. Classification of proteins according to its three-dimensional structure. Alpha proteins. Alpha/beta protein. Protein beta. Structural classes of proteins. CATH classification. SCOP classification. DALI classification. SMART classification. |
| Criteria for the choice of a method of purification and preliminary characterization. | Chromatographic techniques: gel filtration, ion exchange, affinity and hydrophobic interaction. Purification strategies. Preliminary characterization of the protein conformation: State of aggregation, compactness. Secondary structure and tertiary structure indicators. Quantification of proteins. |
| Experimental determination of the structure of proteins using diffraction X. | Crystallization techniques. Tools and strategies for diffraction data. Interpretation of the XRD. Obtaining and refinement of the molecular model. Parameters for calculating the convergence of the model. Modelling. |
| Interactions between biomolecules. | Interactions of proteins for the formation of complexes with proteins and other ligands. Experimental methods used to determine these interactions and their structure. The double hybrid method. The split-ubiquitin method. Pull-down. GST-Pull-down. FRET. EMSA trials. CHIP test. Other methodologies. |
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