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Teaching GuideTerm Faculty of Science |
| Mestrado Universitario en Investigación Química e Química Industrial (Plan 2020) |
 Subjects |
| Molecular Biology |
| Contents |
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| Identifying Data | 2020/21 | |||||||||||||
| Subject | Molecular Biology | Code | 610509117 | |||||||||||
| Study programme |
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| Descriptors | Cycle | Period | Year | Type | Credits | |||||||||
| Official Master's Degree | Yearly |
First | Optional | 3 | ||||||||||
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| Topic | Sub-topic |
| ITEM 1. Handling and DNA sequencing. 1. Sense of the subject (Introduction ) This topic will address the description of the main tools and methods to manipulate genetic information and verify that the operations have been successful and have been conducted as they are designed . |
2. Wording of the subject. Restriction enzymes and cloning vectors hybridization for the detection of specific sequences, PCR, DNA sequencing. 3. Bibliography - Molecular Biology of the Cell fifth edition ( 2008 ) . Alberts et al. Garland Science. Chapter 8. - Molecular Biology fourth edition (2008 ) . Robert F. Weaver. McGraw -Hill International Edition. Chapters 4 and 5. 4. Activities to develop. During the development of the subject practical cases that students must solve using the knowledge gained will arise. It could also be considered that students will read scientific articles related to the topic for exhibition / public presentation. |
| ITEM 2. Techniques for obtaining and analyzing proteins. 1. Sense of the subject (Introduction) We look at how, using the techniques seen in item 1, we can produce and purify proteins in the lab for testing its activity, determine its structure, etc. |
2. Wording of the subject. Recombinant proteins , labeling of proteins, protein expression and purification , and protein sequencing analysis . 3. Bibliography - Molecular Biology of the Cell fifth edition ( 2008 ) . Alberts et al. Garland Science. Chapter 8. - Molecular Biology fourth edition (2008 ). Robert F. Weaver. McGraw -Hill International Edition. Chapters 4 and 5. 4. Activities to develop. During the development of the subject practical cases that students must solve using the knowledge gained will arise. It could also be considered that students will read scientific articles related to the topic for exhibition / public presentation. |
| ITEM 3. Viewing biological processes. 1. Sense of the subject (Introduction) In this issue the main methods of display structures and biological processes, both in vivo and in vitro will be explained. |
2. Wording of the subject. Chemical markers , GFP and fluorescent fusion proteins , optical microscopy (confocal, super-resolution, real-time ), electron microscopy. 3. Bibliography - Molecular Biology of the Cell fifth edition (2008). Alberts et al. Garland Science. Chapter 9. 4. Activities to develop. During the development of the subject practical cases that students must solve using the knowledge gained will arise. Also scientific work to read an comment by the students. |
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