Identifying Data 2015/16
Subject (*) Xenómica Code 610441014
Study programme
Mestrado Universitario en Bioloxía Molecular , Celular e Xenética
Descriptors Cycle Period Year Type Credits
Official Master's Degree 2nd four-month period
First Optativa 3
Language
Spanish
Galician
English
Teaching method Face-to-face
Prerequisites
Department Bioloxía Celular e Molecular
Coordinador
Becerra Fernandez, Manuel
E-mail
manuel.becerra@udc.es
Lecturers
Becerra Fernandez, Manuel
Vila Taboada, Marta
E-mail
manuel.becerra@udc.es
marta.vila.taboada@udc.es
Web
General description Denomínase xenómica ao estudo integral do funcionamento, evolución e orixe dos xenomas. A xenómica utiliza coñemenetos derivados de distintas disciplinas como xenética, bioloxía molecular, bioquímica, informática, estatística, matemáticas e física.
A diferenza da xenética clásica que a partires dun fenotipo (xeralmente mutante) procura o xene ou xenes responsables dese fenotipo, a xenómica ten como obxectivo predicir a función dos xenes a partir da súa secuencia ou das súas interaccións con outros xenes.
As denominadas "ciencias ómicas" están na vangarda da ciencia, feito debido ás posibilidades abertas polas novas tecnoloxías de secuenciación masiva, aos avances en bioinformática e aos algoritmos cada vez máis sofisticados para análise de xenomas completos.

Study programme competencies
Code Study programme competences
A1 Skills of using usual techniques and instruments in the cellular, biological and molecular research: that are able to use techniques and instruments as well as understanding potentials of their uses and applications.
A3 Skills of understanding the functioning of cells through the structural organization, biochemistry, gene expression and genetic variability.
A11 Skills of understanding the structure, dynamics and evolution of genomes and to apply tools necessary to his study.
B1 Analysis skills to understand biological problems in connection with the Molecular and Cellular Biology and Genetics.
B2 Skills of decision making for the problem solving: that are able to apply theoretical knowledges and practical acquired in the formulation of biological problems and the looking for solutions.
B3 Skills of management of the information: that are able to gather and to understand relevant information and results, obtaining conclusions and to prepare reasoned reports on scientific and biotechnological questions
B5 Correct oral and written communication on scientific topics in the native language and at least in another International diffusion language.
B9 Skills of preparation, show and defense of a work.
C3 Skills of Using basic tools of the information technologies and communications (ICT) necessary to the exercise of his profession and for the apprenticeship over his life.
C8 Considering the importance that the investigation has, the innovation and the technological development in the socioeconomic advance and cultural of the society.

Learning aims
Learning outcomes Study programme competences
To learn the basics of the different molecular techniques used in genomics, with particular emphasis in NGS AR1
BR1
BR5
To acquire an updated view about the current scope and future perspectives of structural, functional and evolutionary genomics AR1
BR1
BR5
BR9
CC8
To understand how genomes evolve and how molecular and bioinformatic tools are used for that purpose AR1
AR3
AR11
BR1
BR2
BR9
DNA microarrays: experimental set up and data analysis. AR3
BR1
BR2
BR3
CC3

Contents
Topic Sub-topic
Structural Genomics Mapping, sequencing, annotation and databases
The Human Genome Project
Next Generation Sequencing (NGS)
Comparative Genomics How do genomes evolve?
Genomes of Prokaryotes Metagenomics
Genomes of Eukaryotes Taxonomy
Paleogenomics
Medicine
Functional Genomics DNA microarrays: methodology, types of platforms, experimental set up, data analysis
Computer lab 1. Exploring databases (e.g. ENSEMBL) and large-scale genomic projects (ENCODE, EXOME, 1000Genomes)
2. Using GENEIOUS to assemble an organelle genome from NGS-generated data
3. Gene expression analysis and microarrays

Planning
Methodologies / tests Competencies Ordinary class hours Student’s personal work hours Total hours
Guest lecture / keynote speech A1 A11 B1 10 18.62 28.62
Oral presentation B5 B9 5 3 8
Events academic / information C8 1 3 4
ICT practicals A3 C3 10 20 30
Objective test B2 B3 2 0 2
 
Personalized attention 2.38 0 2.38
 
(*)The information in the planning table is for guidance only and does not take into account the heterogeneity of the students.

Methodologies
Methodologies Description
Guest lecture / keynote speech Active lecturing.
Oral presentation Each student will have to summarize in up to 15 minutes the content of a review paper. Readings assignments will be made available three weeks in advance via MOODLE.
Events academic / information During the term, a seminar speaker, with expertise in Genomics, is invited as a guest lecturer. Students are required to attend this seminar and read the paper assigned (via MOODLE) for this activity.
ICT practicals OUr 10-hour, computer lab curriculum was developed to accompany the lecture course in Genomics. The students work on their own web-based investigations and present their results to each other (active learnning).
Objective test Written exam.

Personalized attention
Methodologies
Oral presentation
ICT practicals
Description
instructors will typically be available via email. Students can arrange for in-person tutoring sessions.

Assessment
Methodologies Competencies Description Qualification
Guest lecture / keynote speech A1 A11 B1 Attendance and active learning.
10
Oral presentation B5 B9 Summarising and communication skills.
20
ICT practicals A3 C3 Attendance and active learning.
Distance learning students will be required to submit a report, so that
comprehension and performance may be assessed.
10
Objective test B2 B3 The exam will evaluate items discussed in the aforementioned activities. 50
Events academic / information C8 Attendance and participation in the final discussion.
10
 
Assessment comments

Mark "A with distinction" will only be awarded to outstanding students passing the subject in June.


Sources of information
Basic McLachlan, G. J., Do, K-A., Ambroise, C (2004). Analyzing Microarray Gene Expression Data. Wiley-Interscience. John Wiley & Sons
Campbell, A.M & Heyer, L.J. (2007). Discovering Genomics, Proteomics & Bioinformatics. Pearson Benjamin Cummings
Bowtell, D., Sambrook, J. (2003). DNA Microarrays. Cold Spring Harbor Laboratory Press.
Allison, David B., et al (2006). DNA microarrays and related genomics techniques design, analysis, and interpretation of experiments. Chapman & Hall/CRC
E. Rinaldis, A. Lahm. (2007). DNA microarrays: current applications. Wymondham: Horizon Bioscience
Lesk, Arthur M. (2012). Introduction to Genomics. Oxford University Press

Complementary Straalen, Nico M. van (2006). An introduction to ecological genomics. Oxford University Press
Zhanjiang, Liu (2007). Aquaculture genome techonologies. Blackwell
Futuyama, Douglas J. (2006). Evolution. Sinauer Associates
Dale Jeremy (2008). From genes to genomes: concepst and applications of DNA technology. John Wiley & Sons
Sensen, Christoph W. (2005). Handbook of genome research genomics, proteomics, metabolism, bioinformatics, ethical & legal issues . Wiley-VCH

RECURSOS EN INTERNET:


Biological database compilation at NAR:
http://nar.oupjournals.org/content/vol29/issue1

DOE Joint Genome Institut. Why sequence them?
http://www.jgi.doe.gov/sequencing/why/index.html

EMBL (European Molecular BIology Laboratory), Bioinformatics.
http://www-db.embl.de/jss/servlet/de.embl.bk.emblGroups.EmblGroupsOrg/serv_0?t=0

ExPASy (Expert Protein Analysis System).
http://us.expasy.org/

GeneMark:
http://opal.biology.gatech.edu/GeneMark/

GenomeNet (Kyoto University Bioinformatics Center).
http://www.genome.jp/

Genoscope. Le séquençage des génomes.
http://www.genoscope.cns.fr/externe/Francais/Sequencage/

GOLD (Genomes Online Database).
http://www.genomesonline.org/

Human genome: advanced annotation tutorial.

http://www.mad-cow.org/00/annotation_tutorial.html

Human Genome Project Information.

http://www.ornl.gov/sci/techresources/Human_Genome/home.shtml

Iañez Pareja, E. (1997). Introducción a los Proyectos Genoma.
http://www.ugr.es/~eianez/Biotecnologia/genoma-2.html

KEGG (Kyoto Encyclopedia of Genes and Genomes).
http://www.genome.jp/kegg/kegg2.html

Nacional Human Genome Research Institute:
http://www.genome.gov/

NCBI (National Center for Biotechnology Information).
http://www.ncbi.nlm.nih.gov/

The Sanger Institute.
http://www.sanger.ac.uk/

TIGR (The Institute for Genomic Research).
http://www.tigr.org/

tRNAscan-SE 1.21.
http://www.genetics.wustl.edu/eddy/tRNAscan-SE/

The WWW Virtual Library: Model Organisms:
http://www.ceolas.org/VL/mo/

ALGUNAS BASES DE DATOS DE MICROARRAYS DE ADN

ArrayExpress

http://www.ebi.ac.uk/arrayexpress/

ChipDB

http://staffa.wi.mit.edu/chipdb/public/

ExpressDB

http://twod.med.harvard.edu/ExpressDB/

Gene Expression Omnibus (GEO)

http://www.ncbi.nlm.nih.gov/geo/

READ (RIKEN cDNA Expression Array Database)

http://read.gsc.riken.go.jp/

Saccharomyces Genome Database (SGD)

http://genome-www4.Stanford.EDU/cgi-bin/SGD/expression/expressionConnection.pl

Standford Microarray Database ( SMD)

http://genome-www5.stanford.edu/

The Yale Microarray Database

http://www.med.yale.edu/microarray/

yeast Microarray Global Viewer (yMGV)

http://www.transcriptome.ens.fr/ymgv/


Recommendations
Subjects that it is recommended to have taken before
Técnicas Celulares/610441001
Técnicas Moleculares/610441002
Mecanismos de xeración da variación xenética/610441005
Regulación da expresión xénica/610441006
Bioinformática e Modelado de Biomoléculas/610441020

Subjects that are recommended to be taken simultaneously
Proteómica/610441013
Cromosomas: Estructura. Función e Evolución/610441015
Xenética Humana/610441016
Toxicología Xenética/610441017

Subjects that continue the syllabus
Traballo de Máster/610441022

Other comments


(*)The teaching guide is the document in which the URV publishes the information about all its courses. It is a public document and cannot be modified. Only in exceptional cases can it be revised by the competent agent or duly revised so that it is in line with current legislation.