Study programme competencies |
Code
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Study programme competences / results
|
A1 |
Skills of using usual techniques and instruments in the cellular, biological and molecular research: that are able to use techniques and instruments as well as understanding potentials of their uses and applications. |
A2 |
Skills of working in a sure way in the laboratories knowing operation handbooks and actions to avoid incidents of risk. |
A3 |
Skills of understanding the functioning of cells through the structural organization, biochemistry, gene expression and genetic variability. |
A4 |
Skills to apply molecular techniques to the study of the plant cell physiology, its response to external triggers and their biotechnological applications. |
A5 |
Skills of understanding the microorganisms' role as pathogenic agents and as biotechnological tools. |
A8 |
Skills of having an integrated view of the previously acquired knowledge about Molecular and Cellular Biology and Genetics, with an interdisciplinary approach and experimental work. |
A9 |
Skills of understanding the structure and dynamics of proteins to individual and proteomic level, as well as the techniques that are necessary to analyze them and to study their interactions with other biomolecules. |
A10 |
Skills of modifying genes, proteins and chromosomes with biotechnological applications |
A12 |
Skills to understand, detect and analyze the genetic variation, knowing genotoxicity processes and methodologies for its evaluation, as well as carrying out diagnosis and genetic risk studies. |
A13 |
Skills to become a professional in health, pharmacy, veterinary, animal production, biotechnology or food sectors. |
B1 |
Analysis skills to understand biological problems in connection with the Molecular and Cellular Biology and Genetics. |
B2 |
Skills of decision making for the problem solving: that are able to apply theoretical knowledges and practical acquired in the formulation of biological problems and the looking for solutions. |
B3 |
Skills of management of the information: that are able to gather and to understand relevant information and results, obtaining conclusions and to prepare reasoned reports on scientific and biotechnological questions |
B4 |
Organization and work planning skills: that are able to manage the use of the time as well as available resources and to organize the work in the laboratory. |
C6 |
Considering critically the knowledge, technologies and the available information to solve problems with which should face. |
Learning aims |
Learning outcomes |
Study programme competences / results |
Handle the necessary equipment for cellular and molecular techniques. |
AR1 AR2
|
|
|
Know the protocols used for the different techniques. |
AR1 AR2
|
|
|
Know the applications for the different techniques. |
AR1 AR4 AR5 AR13
|
BR2
|
CC6
|
Consider the ways to resolve the methodological problems associated with the performance of the techniques. |
|
BR1
|
|
Establish the relationships between the different techniques used and its possible combination to resolve the problems. |
|
BR1
|
|
Interpret data from observations and measurements in the laboratory. |
|
BR3
|
|
Plan, design and conduct experiments related with the techniques learned. |
|
BR2 BR4
|
|
Maintain a critical attitude for a perfect experimental work. |
|
|
CC6
|
Relate the chemical and structural properties of biomolecules with laboratory techniques that are most suitable for isolation, purification and characterization. |
AR1 AR9
|
BR1 BR2
|
|
Know in depth the possibilities and characteristics of PCR and real-time PCR. |
AR2
|
BR3 BR4
|
|
Understand and handle the techniques of recombinant DNA that can be used for analysis and manipulation of biomolecules. |
AR1 AR2 AR8 AR10
|
BR2
|
|
Use methods and techniques to detect and analyze genetic variation. |
AR1 AR3 AR12
|
BR3
|
|
Contents |
Topic |
Sub-topic |
Purification of Biomolecules |
Principle of centrifugation technique and instrumentation. Preparative and Analytical Centrifugation.
Chromatographic Techniques: principle and selection criteria.
Electrophoresis: principle and types. Isoelectric focusing technique. Capillary electrophoresis. |
PCR |
Advanced concepts in PCR
Differences between PCR and Real-time PCR
Detection methods of amplicons
Trial design and results analysis |
Tecnology of molecular markers |
Concept and single nucleotide polymorphisms (SNPs)
Protein markers
DNA markers based in Nucleic Acid Hibridization
Pattern Multi Locus by PCR techniques
DNA markers based in PCR Mono-locus
Single nucleotide polymorphisms (SNPs) |
Recombinant DNA |
Enzymes and protocols used in recombinant DNA techniques
Genomics GeneBank
Expression GeneBank
GeneBank analysis
Transfer and Blotting techniques
Sequencing techniques
Site-direct mutagenesis techniques
Silencing techniques
Transgenic organisms: uses and applications |
Planning |
Methodologies / tests |
Competencies / Results |
Teaching hours (in-person & virtual) |
Student’s personal work hours |
Total hours |
Guest lecture / keynote speech |
A1 A4 A5 A10 A13 |
14 |
14 |
28 |
Laboratory practice |
A1 A2 A3 A12 B4 |
24 |
48 |
72 |
Supervised projects |
A1 A3 A8 A9 B1 B3 B2 |
0 |
42 |
42 |
Mixed objective/subjective test |
A1 A3 A9 A12 B1 B2 C6 |
2 |
4 |
6 |
|
Personalized attention |
|
2 |
0 |
2 |
|
(*)The information in the planning table is for guidance only and does not take into account the heterogeneity of the students. |
Methodologies |
Methodologies |
Description |
Guest lecture / keynote speech |
By the Professors or/and by the exhibition of student work |
Laboratory practice |
Practical classes in the laboratory; Problems solving and practical cases |
Supervised projects |
Research Project related with the techniques made in the laboratory. It will be develop individually under Professor’s supervisión.
|
Mixed objective/subjective test |
Exam about theoretical and practical subjects. |
Personalized attention |
Methodologies
|
Supervised projects |
Guest lecture / keynote speech |
Laboratory practice |
|
Description |
Personalized tutoring focused on guidance to help the students: resolving doubts and clarifications.
The tutoring schedule will be indicated the first class by each Professor. The students may request an appointment and/or resolving doubts by e-mail.
|
|
Assessment |
Methodologies
|
Competencies / Results |
Description
|
Qualification
|
Supervised projects |
A1 A3 A8 A9 B1 B3 B2 |
Elaboration and writing of a supervised work. |
30 |
Laboratory practice |
A1 A2 A3 A12 B4 |
Along the practical classes, the students will answer questions and problems, which will be part of the continuous evaluation of the course. |
20 |
Mixed objective/subjective test |
A1 A3 A9 A12 B1 B2 C6 |
Exam with questions in which the student must apply the knowledge and skills acquired along the course.
|
50 |
|
Assessment comments |
.-The evaluation criteria listed will be apply to two types of registration (classroom and blended learning).
.-The attendance to Practical clases is a necessary condition to be evaluated.
.-The qualifications obtained with the Supervised Project and Practical Exercises will be maintained for the 2º Option (July) if the student do not pass the Final Exam in the 1º Option (January), and in the Final Qualification Records (QRs) will appear the qualification of 4.
.-According to the rule of qualifications and records in Grades and Masters, the Quality Committee of the Faculty of Sciences, agreed to the recommendation to concede the “Honors Qualification” to those students who obtained the highest marks in the 1st Option_June.
|
Sources of information |
Basic
|
M. L. Marina, A. Ríos, M. Valcárcel (2005). Analysis and detection by capillary electrophoresis . Amsterdam : Elsevier
Westermeier, Reiner. (2005). Electrophoresis in practice : a guide to methods and applications of DNA and protein separations. Weinheim : Wiley-VCH
Weiner MP, Gabriel SB, Stephens JC, (2007). Genetic variation: a laboratory manual. Cold Spring harbor Laboratory Press, New York.
Brown TA (2008). Genomes (3º ed). . Médica Panamericana, Buenos Aires.
Morteza G. Khaledi (1998). High-performance capillary electrophoresis theory, techniques, and applications . New York : John Wiley & Sons,
Nuez F, Carrillo JM, (2000). Los marcadores genéticos en la mejora vegetal.. Universidad Politécnica de Valencia.
Avise CJ (2004). Molecular markers, natural history, and evolution (2ª ed.). . Sinauer Associates, Sunderland, MA.
Keith Wilson and John Walker (1995). Principles and Techniques of Practical Biochemistry. Cambridge, University Press
Dorak, T. (2007). Real-Time PCR. Routledge Taylor and Francis.
Mackay, I. M. (2007). Real-time PCR in microbiology : from diagnosis to characterisation. Norfolk: Caister Academic Press.
Edwards, K., Logan J. & Saunders, N. (2004). Real-time PCR: an essential guide.. Horizon bioscience.
Logan J, Edawards K, Saunders N. (2009). Real-Time PCR: Current Technology and applications.. Caister Academic Press |
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Complementary
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In addition, it will be provide research articles on the topics covered during the course in the Moodle virtual platform. |
Recommendations |
Subjects that it is recommended to have taken before |
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Subjects that are recommended to be taken simultaneously |
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Subjects that continue the syllabus |
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