Study programme competencies |
Code
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Study programme competences
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A5 |
Analizar e caracterizar mostras de orixe humana. |
A11 |
Identificar e analizar material de orixe biolóxica e as súas anomalías. |
A12 |
Manipular material xenético, realizar análises xenéticas e levar a cabo asesoramento xenético. |
A15 |
Deseñar e aplicar procesos biotecnológicos. |
A29 |
Impartir coñecementos de Bioloxía. |
A30 |
Manexar adecuadamente instrumentación científica. |
A31 |
Desenvolverse con seguridade nun laboratorio. |
B1 |
Aprender a aprender. |
B2 |
Resolver problemas de forma efectiva. |
B3 |
Aplicar un pensamento crítico, lóxico e creativo. |
B5 |
Traballar en colaboración. |
B7 |
Comunicarse de maneira efectiva nunha contorna de traballo. |
Learning aims |
Learning outcomes |
Study programme competences |
General knowledge and understanding of the molecular basis of the organization, expression, variation and manipulation of genetic material. |
A11 A12 A15 A29
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B1 B2 B3 B5 B7
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Knowledge of the basic methodologies used in Molecular Genetics. |
A5 A11 A12 A15 A29 A30 A31
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B1 B2 B3 B5
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Ability to use sources of information of interest in Molecular Genetics. |
A5 A11 A12 A15 A29
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B1 B2 B3
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Ability to interpret and transmit Molecular Genetics information. |
A29
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B1 B2 B3 B5 B7
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Contents |
Topic |
Sub-topic |
1.- DNA REPLICATION |
Semiconservative DNA replication: the Meselson and Stahl experiment. Modes of replication. Enzymology of the replication. DNA replication in Escherichia coli. DNA replication in eukaryotes. Telomere synthesis. Replication of mitochondrial and chloroplast DNA.
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2.- SYNTHESIS AND PROCESSING OF RNA |
Classes of RNA. RNA polymerases. Promoters and transcriptional apparatus. Transcription in prokaryotes and eukaryotes: initiation, elongation and termination. Interrupted genes: exons and introns. Processing of eukaryotic pre-mRNA. Synthesis and processing of pre-rRNA. Synthesis and processing of pre-tRNA. RNA edition. Revision of gene concept.
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3.- TRANSLATION |
Central dogma in molecular biology. Ribosomes and tRNAs. Translation cycle: initiation, elongation, and termination. Genetic code and genetic decoding. Peptydil transferase reaction. The ribosome: composition. Phylogenetic conservation of rRNA. Role of rRNA in initiation. Role of RNA in decoding. Role of RNA in peptydil transfer. The hypothesis of the RNA world. |
4.- MUTATION AND DNA REPAIR |
Molecular basis of spontaneous mutations: replication errors, unequal crossing over, spontaneous chemical changes. Molecular basis of induced mutations: chemical and physical agents. Repair mechanisms: direct reversal of damaged DNA, excision repair, mismatch repair, repair of double-strand breaks, translesion synthesis.
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5.- MOLECULAR MECHANISM OF GENETIC RECOMBINATION
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The role of genetic recombination. Gene conversion. Models of homologous recombination: Holliday model and double-strand break model. Enzymes required for recombination. Site-specific recombination. Immunoglobulin gene rearrangements.
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6.- TRANSPOSABLE GENETIC ELEMENTS |
Transposable elements in prokaryotes: insertion sequences, composite transposons and noncomposite transposons. Replicative and non replicative transposition. Transposable elements in eukaryotes: transposons and retrotransposon. Evolutionary significance of transposable elements.
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7.- RECOMBINANT DNA TECHNOLOGY |
Restriction enzymes. Cloning vectors. DNA libraries: construction and screening. Southern and northern blotting. PCR. Restriction maps. DNA sequencing. Site-directed mutagenesis.
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8.- APPLICATIONS OF RECOMBINANT DNA TECHNOLOGY
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Expression of eukaryotic genes in E. coli. DNA transfer to eukaryotic cells. Transgenic animals. Transgenic plants. Gene therapy. Genetic diagnosis. Genome editing: CRISPR/Cas9 technology.
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9.- GENOMICS
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Structural genomics: molecular markers and genetic maps. DNA fingerprinting. Structural genomics: physical maps and genome annotation. Functional genomics: DNA microarrays, RNA-seq and reverse genetics. Comparative genomics. Metagenomics. Synthetic biology.
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10.- REGULATION OF GENE EXPRESSION IN BACTERIA
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Jacob and Monod’s operon model for the regulation of lac genes in E. coli. Positive control of the lac operon. The arabinose operon of E. coli: positive and negative control. The triptophan operon of E. coli: negative control and attenuation. RNA-mediated regulation. |
11.- REGULATION OF GENE EXPRESSION IN EUKARYOTES
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Changes in chromatin structure. DNA methylation. Transcriptional control.
RNA processing control. Control of mRNA stability. Control at the level of translation. RNA interference. Epigenetics.
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PRACTICE 1: DNA EXTRACTION |
Genomic DNA extraction. Agarose gel electrophoresis for DNA. DNA quantification. |
PRACTICE 2: PCR |
PCR amplification of the CHD gene. Analysis of an intron polymorphism for bird sexing. |
PRACTICE 3: DOT-BLOT |
Nucleic acids hybridization: detection of microsatellite sequences by dot-blot |
PRACTICE 4: BIOINFORMATICS. |
Analyses and comparison of nucleic acid sequences. Primer design. |
Planning |
Methodologies / tests |
Competencies |
Ordinary class hours |
Student’s personal work hours |
Total hours |
Guest lecture / keynote speech |
A5 A11 A12 A15 B2 B3 B7 |
28 |
42 |
70 |
Seminar |
A5 A11 A12 A15 A29 B1 B2 B3 B5 B7 |
8 |
12 |
20 |
Laboratory practice |
A5 A11 A12 A15 A30 A31 B1 B2 B3 B5 B7 |
15 |
7.5 |
22.5 |
Supervised projects |
A5 A11 A12 A15 A29 B1 B2 B3 B5 B7 |
0 |
29.5 |
29.5 |
Mixed objective/subjective test |
A5 A11 A12 A15 A29 B1 B2 B3 B7 |
6 |
0 |
6 |
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Personalized attention |
|
2 |
0 |
2 |
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(*)The information in the planning table is for guidance only and does not take into account the heterogeneity of the students. |
Methodologies |
Methodologies |
Description |
Guest lecture / keynote speech |
The teacher explains the main contents of each lesson. |
Seminar |
Resolution/discussion of questions and problems related to the subject. |
Laboratory practice |
The student conducts laboratory experiences following a protocol, under the supervision of the teacher. |
Supervised projects |
Solving of a practical case related to Bioinformatics, as well as, three questionnaires with exercises and questions related to some aspect of the subject. Both activities are done in groups. The practical case shall be delivered in writing. |
Mixed objective/subjective test |
Different types of questions (essay, multiple-choice, short answer, and multiple-matching) and problem solving. |
Personalized attention |
Methodologies
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Supervised projects |
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Description |
Individually or in group, students can attend tutorial sessions to consult any doubts that might arise from the different activities. |
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Assessment |
Methodologies
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Competencies |
Description
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Qualification
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Supervised projects |
A5 A11 A12 A15 A29 B1 B2 B3 B5 B7 |
Assessment will be based on the proportion of correct answers, the clarity of the explanations, the quality of the presentation, and the reasoning used in answers. The practical case represents 20% of the final grade and will be graded at the group level by means of the delivered written (10%) and at the individual level (10%). Questionnaires represent 10% of the final grade and will be graded at the individual level only. Individual evaluations will be carried out through a short answer test (phrase, word, number or symbol), which could be face to face or via Moodle. |
30 |
Mixed objective/subjective test |
A5 A11 A12 A15 A29 B1 B2 B3 B7 |
The degree of general knowledge and understanding of the subject will be assessed.
It will consist of two parts.
One is related to the theoretical content and represents 60% of the score.
The other is related to the laboraroty practices and represents 10% of the score.
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70 |
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Assessment comments |
To pass the course, the score must be 5 or higher, but at least a 4 is required in all tests corresponding to theory, laboratory practices, and practical case. If the sum of the score of all activities is higher than 5, but the score on one the tests is lower than 4, the final score is 4.9 (failing score). The grade of Non Attendance (NP) will be applied to the students that do no attend the official exam. Preferably, first class honors will be awarded in January among students with a score of 9 or higher. A mid-term exam will be held. A score of 4 or higher will be maintained until July. In July there will be a second opportunity to take the tests corresponding to theory, laboratory practices, and practical case. First opportunity's scores of questionnaires and group grades of practical case will be maintained. In the case of justified exceptional circumstances, additional measures may be taken, so that the student can pass the subject, such as flexibility in the delivery date of supervised projects, flexibility in practice schedules or a global assessment test of the learning results.
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Sources of information |
Basic
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Klug, W.S., Cummings, M.R., Spencer, C.A (2013). Conceptos de Genética . Pearson/Prentice Hall, Madrid
Pierce, B.A. (2010). Genética: un enfoque conceptual. Médica Panamericana, Madrid |
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Complementary
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Lodish, H., Berk, A., Kaiser, C.A., Krieger, M., Bretscher, A., Ploegh, H., Amon, A., Scott, M.P. (2016). Biología celular y molecular (7ªed) . Médica Panamericana, Madrid
Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K., Walter, P. (2010). Biología molecular de la célula. Omega, Barcelona
Herráez, A. (2012). Biología molecular e ingeniería genética. Elsevier, Ámsterdam
Lewin, B. (2008). Genes IX. McGraw-Hill. México
Benito, C., Espino, F.C. (2013). Genética: conceptos esenciales. Médica Panamericana, Madrid
Snustad, D.P., Simmons, M.J. (2012). Genetics (6th ed.). John Wiley and Sons, New York
Brooker, R.J. (2018). Genetics: analysis and principles (6th ed.). McGraw-Hill, New York
Hartwell, L.H., Goldberg, M.L., Fischer, J.A., Hood, L., Aquadro, C.F. (2015). Genetics: from genes to genomes (5th ed.) . McGraw-Hill, New York
Brown, T.A. (2008). Genomas (3ª ed.). Médica Panamericana, Buenos Aires
Russell, P.J. (2010). iGenetics: a molecular approach (3rd ed.) . Benjamin Cummings, San Francisco
Perera, J., Tormo, A., García, J.L. (2002). Ingeniería genética. Vol. I: Preparación, análisis, manipulación y clonaje de DNA. Síntesis, Madrid
Perera, J., Tormo, A., García, J.L. (2002). Ingeniería genética. Vol. II. Expresión de DNA en sistemas heterólogos. Síntesis, Madrid
Griffiths, A.J.F., Wessler, S.R., Carroll, S.B., Doebley, J. (2015). Introduction to genetic analysis (11th ed.). W.H. Freeman, New York
Krebs, J.E., Goldstein, E.S., Kilpatrick, S.T. (2012). Lewin genes: fundamentos. Médica Panamericana, Madrid
Watson, J.D., Baker, T.A., Bell, S.P., Gann, A., Levine, M., Losick, R. (2014). Molecular biology of the gene. Pearson, Boston
Cox, M.M., Doudna, J.A., O'Donnell (2012). Molecular biology: principles and practice. W.H. Freeman, New York
Craig, N.L., Cohen-Fix, O., Green, R., Greider, C., Storz, G., Wolberger, C. (2014). Molecular biology: principles of genome function. Oxford University Press, Oxford |
Consult the Moodle platform for additional sources of information. |
Recommendations |
Subjects that it is recommended to have taken before |
Biology: Basic Levels of Organisation of Life I (Cells)/610G02007 | Biochemistry I/610G02011 | Biochemistry II/610G02012 | Microbiology/610G02015 | Genetics/610G02019 |
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Subjects that are recommended to be taken simultaneously |
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Subjects that continue the syllabus |
Population Genetics and Evolution/610G02021 | Cytogenetics/610G02022 |
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Other comments |
Recommendations:
Attend class and follow the development of the course regularly.
Check Moodle and email regularly to obtain the materials and know the schedule of activities.
Attend tutorials to resolve any questions or difficulties that may arise.
Consult the recommended bibliography.
Keep up-to-date with course work. |
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