Study programme competencies |
Code
|
Study programme competences / results
|
A8 |
Illar, analizar e identificar biomoléculas. |
A12 |
Manipular material xenético, realizar análises xenéticas e levar a cabo asesoramento xenético. |
A17 |
Realizar bioensaios e diagnósticos biolóxicos. |
A27 |
Dirixir, redactar e executar proxectos en Bioloxía. |
A29 |
Impartir coñecementos de Bioloxía. |
A30 |
Manexar adecuadamente instrumentación científica. |
A31 |
Desenvolverse con seguridade nun laboratorio. |
B1 |
Aprender a aprender. |
B2 |
Resolver problemas de forma efectiva. |
B3 |
Aplicar un pensamento crítico, lóxico e creativo. |
B4 |
Traballar de forma autónoma con iniciativa. |
B5 |
Traballar en colaboración. |
B7 |
Comunicarse de maneira efectiva nunha contorna de traballo. |
B10 |
Exercer a crítica científica. |
B11 |
Debater en público. |
B13 |
Comportarse con ética e responsabilidade social como cidadán e como profesional. |
Learning aims |
Learning outcomes |
Study programme competences / results |
The approach of the Master Classes is to improve the knowledge and the ability of reflection on a discipline that also, once in the professional field, will demand a good praxis and adhere to ethical principles. The Laboratory Classes are more focused on the expertise on know how to do and how to be, related to the field of the Biochemistry and Molecular Biology. |
A8 A12 A17 A27 A29 A30 A31
|
B1 B2 B3 B4 B5 B7 B10 B11 B13
|
|
Contents |
Topic |
Sub-topic |
1.-Basal Transcription |
RNA polymerases, core promoter and general transcription factors.Transcription mechanism: initiation, elongation and termination. Methodology to study: transcription start site selection, transcriptional termination and interactions nucleic acids-proteins. |
2.-Regulated transcription and chromatin involvement in transcriptional regulation |
Activators and repressors. DNA binding domains: DNA-proteins interactions. Chromatin remodeling complexes. Acetilation, deacetilation and other histones modifications in the regulation of gene expression. Techniques to study transcriptional regulation. Regulation examples of specific genes |
3.-RNA processing and coordination of co-transcriptional events in eukaryotes |
RNA cleavage and polyadenylation. RNA splicing. Processing of ribosomic and transferent RNA |
4.-RNA as regulator of gene expression |
RNA edition. Control of mRNA quality. Function of snRNA and transcriptional regulation. sncRNAs and the gene silencing mechanism. Antisense RNA in the translational regulation and applications of RNA. RNomics aspects |
5.-Protein Translation |
General aspects. Ribosomes. Translation mechanism: Initiation, elongation and termination. Differences in eukaryotes. Translation in mitochondria. Translational inhibitors. |
6.-Protein Processing |
Postranslational modifications of proteins. Folding: Chaperones and Prions. Ubiquitination and SUMOilation. Programed degradation: Proteasome |
7.-Protein Transport |
Cotranslational and postranslational translocation. Classification and distribution of new synthesized proteins. Traffic nucleo-cytoplasm. Transport regulation and final destiny of proteins in the cell. |
8.-Basics of Cell Signaling
|
Classification of intercellular communication. Stages of intracellular signaling. Organization of signaling and pathways. Signaling molecules: types and functions. |
9.- Reception of external signals and intracellular transduction |
Membrane and intracellular receptors: types and mechanisms of activation. Intercellular Messenger substances or Second Messengers, protein kinase cascades and signal transduction to nucleus. |
10.- Examples of control mechanisms and coordination of cell physiological activities |
Cell Growth and Proliferation: regulation of cell cycle, apoptosis and cancer. Cell Senescence Signaling. |
Planning |
Methodologies / tests |
Competencies / Results |
Teaching hours (in-person & virtual) |
Student’s personal work hours |
Total hours |
Laboratory practice |
A8 A12 A17 A27 A30 A31 B1 B2 B3 B4 B5 B7 B10 B13 |
15 |
22.5 |
37.5 |
Problem solving |
A29 B1 B2 B3 B4 B5 B7 B10 B11 B13 |
7 |
17.5 |
24.5 |
Guest lecture / keynote speech |
A29 B2 B3 B4 B7 B10 B11 B13 |
28 |
0 |
28 |
Document analysis |
A29 B1 B3 B5 B7 B10 B11 B13 |
1 |
3 |
4 |
Mixed objective/subjective test |
A29 B2 B3 B7 B10 B13 |
2.5 |
50 |
52.5 |
|
Personalized attention |
|
3.5 |
0 |
3.5 |
|
(*)The information in the planning table is for guidance only and does not take into account the heterogeneity of the students. |
Methodologies |
Methodologies |
Description |
Laboratory practice |
Focused on the study of gene expression, with the use of databases, with the analysis of reporter genes expression and/or with the study of protein expression. |
Problem solving |
This section will include the approach and resolution of problems of different aspects in small groups of students, combining the methodologies of problem-based learning and collaborative work. |
Guest lecture / keynote speech |
Oral Presentation complemented with audiovisual media to transmit knowledges and provide the learning. Besides it will improve the participation of the students. |
Document analysis |
Read and comprenhesion of research papers. Searches of information, analysis, discussion, participation of all group components documents elaboration and conclussions. Defense in seminars. |
Mixed objective/subjective test |
It will be used for the evaluation of the knowledge, skills, attitudes, and so, acquired by the student along the course, and will include different types of questions: multiple answer, short, etc. |
Personalized attention |
Methodologies
|
Laboratory practice |
Problem solving |
Guest lecture / keynote speech |
Document analysis |
|
Description |
The tasks to perform by the student will be guided by the Professor. It is important the regular attendance to Tutorials with the Professor, who will help to monitor the progress of the students.
The specific tutorial Schedule for students will be given at the begining of the course. Apart from that, students can e-mail the professors to solve specific questions or (with the teacher´s agreement) to make tutorial appointments or by Teams. |
|
Assessment |
Methodologies
|
Competencies / Results |
Description
|
Qualification
|
Laboratory practice |
A8 A12 A17 A27 A30 A31 B1 B2 B3 B4 B5 B7 B10 B13 |
LABORATORY CLASSES: The assistance is mandatory. The students will interpret the obtained results. Besides they will present a work that will include a small research project based on the results in the practical course and an exam.
|
25 |
Mixed objective/subjective test |
A29 B2 B3 B7 B10 B13 |
FINAL EXAMINATION: The knowledges obtained by the students in the Master and Small Group Classes will be evaluated in a final exam.
|
40 |
Problem solving |
A29 B1 B2 B3 B4 B5 B7 B10 B11 B13 |
Resolution of problems, student's work in Reduced Groups: exercises, seminars (as indicated in document analyses) and possibility of small exams.
|
20 |
Document analysis |
A29 B1 B3 B5 B7 B10 B11 B13 |
Small group activity: Activity of searching and handling of scientific information that will be used to elaborate activities of science dissemination, with a class exposition in small groups, and with an ending debate. The scientific rigor of information (group work), the quality of visual presentation (group work), the fluidity and clarity of oral exposure (individual work) and responsiveness (individual work) will be evaluated.
|
15 |
|
Assessment comments |
.- In order to add all the qualifications and pass this subject it will be mandatory to acquire the minimum scores in the three evaluable parts INDEPENDENTLY: Activities (Problem solving / Document analysis), Practical Classes in the Laboratory and Objective Probe (or Final Examination).
The oficial final exams in June and in July will be presentially, unless in case of pandemic situation in which will use the Moodle platform only.
The continous assesments, previous to the oficial opportunities, will also be on line. Two partial exams will be issued.- For Final Qualifications or ACTAS (on any of the 2 opportunities, JUNE or JULY): The marks of the Objective Probe, Laboratory Practices, and Seminars will be added only if all of them reach 45% of their value. If this percentage is not reached, the final grade that will appear in ACTAS will be 4.
.-In the Final Exam of the 2nd Opportunity_(July), the student will be able to recover only the
theoretical. It will not be an exam for the Practical Part in this 2º Opportunity.
.-The attendance to Practical Laboratory Classes is a mandatory condition to be evaluated. Failure
to attend classes without a properly justified reason means failing the subject. The students who had passed the Practical Part in the two previous academic years may apply for a request for his validation as overcome (PASS). .- The students that do not show up in any of the two official examination dates will obtain a NOT
PRESENTED in the Final Grades (ACTAS).
.-According to the rule of qualifications and records in Degrees and Masters, the Quality Committee of the Faculty of Sciences agreed to the recommendation to concede the “Honors Qualification” to those students who obtained the highest marks in the 1st Opportunity.
.-For students with part-time dedication or with an exemption of class assistance, in June and July, there will be a specific exam for overall assessment.
.- Exceptionally, in the case of those students that, for duly justified reasons, are not able to
perform all continuous assessment tests or evaluable activities, the professors will take their deems for appropriate measures.
.-Students with recognition of part-time dedication and academic exemption for attendance both in the end-of-term opportunity and in the second opportunity will take into account, for the calculation of the overall grade, the grade obtained in the theoretical exam and the corresponding practical part (see above format of both exams), representing these 75% and 25% of the final grade, respectively.
|
Sources of information |
Basic
|
Lodish, Berk, Matsudaria, Kaiser et al., (2008). Biología Celular y Molecular. Ed. Médica Panamericana
Karp G. (2011). Biología Celular y Molecular. Conceptos y experimentos. McGraw-Hill Interamericana Eds., S.A. de C.V., traducción de la 6ª ed. de Cell and Molecular Biology
Elliot, W.H. & Elliot, D.C. (2002). Bioquimica y Biologia Molecular. Ariel, S.A.
Stryer,L, Berg, J.M. %Tymoczko, J.L. (2013). Bioquímica: con aplicaciones clínicas. Ed. Reverté, 7ª Ed.
Lewin B. (2011). Genes X. Jones and Bartlett Publishers, LLC
Bruce, Alberts [et al.]. (2008). Molecular biology of the cell. New York : Garland Science, 5th ed.
Lodish, Berk, Krieger, Kaiser et al., (2013). Molecular Cell Biology. WhFreeman
Whitford, D. (2005). Proteins: Structure and Function. John Wiley & Sons, Ltd.
Meister G. (2011). RNA Biology. Wiley-VHH
Herráez, A. (2012). Texto inlustrado de Biología Molecular e ingeniería genética. Elsevier |
In the Moodle we Will update links to web pages and other bibliographic sources.
Contingency plan: no modifications since they are included in the Moodle. |
Complementary
|
Krauss, Gerhard. (2008). Biochemistry of signal transduction and regulation.. Weinheim : Wiley-VCH. 2nd ed.
Rhoads R. (2010). miRNA Regulation of the translational machinery. Springer
Dalbey, R.E. & von Heijne, G. (2002). Protein targeting, transport & translocation. Academic Press
Meyers, R.A. (2007). Proteins: from analytical to structural genomics (Volume I and II). Wiley-VCH Verlag GmbH & Co. |
|
Recommendations |
Subjects that it is recommended to have taken before |
Bioquímica I/610212101 | Bioquímica II/610212202 | Molecular Genetics/610G02020 |
|
Subjects that are recommended to be taken simultaneously |
|
Subjects that continue the syllabus |
Fundamentos Bioquímicos de Biotecnoloxía/610212620 |
|
Other comments |
.-It is recommended to attend both group and individual tutoring to get best results.
Green
Campus Science Faculty Program
To
contribute to achieving an immediate sustainable environment and comply with
point 6 of the "Environmental Declaration of the Faculty of Sciences
(2020)", the documentary work carried out in this area:
a. They
will be requested mainly in virtual format and computer support.
b. To do on
paper:
- Plastics will
not be used.
-
Double-sided prints will be made.
- Recycled
paper will be used.
- Drafts will be avoided. |
|