Study programme competencies |
Code
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Study programme competences / results
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A8 |
Illar, analizar e identificar biomoléculas. |
A12 |
Manipular material xenético, realizar análises xenéticas e levar a cabo asesoramento xenético. |
A13 |
Realizar o illamento e cultivo de microorganismos e virus. |
A14 |
Desenvolver e aplicar produtos e procesos de microorganismos. |
A15 |
Deseñar e aplicar procesos biotecnológicos. |
B2 |
Resolver problemas de forma efectiva. |
B5 |
Traballar en colaboración. |
B6 |
Organizar e planificar o traballo. |
B8 |
Sintetizar a información. |
Learning aims |
Learning outcomes |
Study programme competences / results |
Coñecer as técnicas actuais de Bioloxía Molecular, Enxeñaría metabólica e de proteínas e as súas principais aplicacións. |
A8 A12 A13
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B2
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Involucrarse na problemática e oportunidades relacionadas co manexo da biotecnoloxía. |
A14 A15
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B5 B6 B8
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Integración dos coñecementos adquiridos en forma separada doutras materias cursadas no tres primeiros anos, con forte exercicio do pensamento crítico. |
A8 A12 A13
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B2 B5 B6 B8
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Amplo dominio da linguaxe, as técnicas e as aplicacións da Bioloxía molecular e da Biotecnoloxía. |
A14 A15
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B2 B5 B6 B8
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Desenvolvemento da percepción das oportunidades que poden derivarse da aplicación de novas estratexias biotecnolóxicas. |
A14 A15
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B2 B5 B6 B8
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Contents |
Topic |
Sub-topic |
B1T1.- INTRODUCTION
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Current concept of biotechnology. History and development of biotechnology. Perspectives. |
B1T2.- BIOTECHNOLOGY OF THE FERMENTATION |
Classification of microbial fermentations. The traditional fermentation industries. Examples. The operating mode in fermentation processes. |
B1T3.- GENES CLONATION |
Purposes of the molecular cloning. Basic stages of the gene cloning. To obtain the DNA. DNA fragmentation: restriction enzymes. Union of DNA molecules. Basic techniques: electrophoresis and hybridization. |
B1T4.- CLONATION VECTORS |
Concept of DNA vector. Organization of the vectors and types
|
B1T5.- LIBRARY |
Concept of library. Gene banks of genomic DNA. cDNA gene banks. Gene banks of expression. Amplification, storage and replication of gene banks. Techniques for the identification of clones. Strategies to confirm the validity of the clone. DNA microarrays. |
B1T6.- TRANSFORMATION |
Transformation systems. Selection of recombinants. Gene expression and its amplification. |
B1T7.- POLYMERASE CHAIN REACTION |
Fundamentals of the method. Automation. Components and reaction conditions. The design of primers. Fidelity of the reaction. Polymerases. Major variants and their applications |
B1T8.- PRODUCTION OF HETEROLOGOUS PROTEINS IN BACTERIA AND YEATS |
Selection of micro-organisms. Vectors of expression and/or secretion. Expression in transformed cells. Secretion. Stability. The process of recovery and downstream purification. Industrial applications. Examples. |
B1T9.-PRODUCTION OF HETEROLOGOUS PROTEINS IN ANIMAL CELLS |
Genetic manipulation of animal cells. Vectors of expression and production of proteins in mammalian cells. Expression of proteins mediated by baculovirus in insect cell cultures. Comparison of the industrial production of heterologous proteins in cultures of bacteria, yeasts and animal cells. |
B1T10.- TRANSGENIC ANIMALS |
Introduction of modified genes into the genome. Pure transgenic and transgenic chimera. Homologous recombination. Specific regulation of transgenes. Gene inactivation. Antisense RNA. Ribozymes. Cell ablation. Applications as study models. Commercial applications. Cloning in mammals. |
B1T11.- GENETIC ENGINEERING OF PLANTS |
In vitro cultures. Techniques of manipulation. The use of markers in diagnosis, improvement and conservation of biodiversity. Resistance of plants against infections, stress and pests. Plants producing proteins of economic interest. Transgenic plant field trials. |
B2T1.-APPLICATIONS OF ENZYMES IN BIOTECHNOLOGY PROCESSES |
Historical perspective. The development of the enzyme industry. |
B2T2.- PRODUCTION OF ENZYMES TO INDUSTRIAL SCALE |
Source selection of the enzyme. New methods of screening. Extremophiles. Post-fermentation processing. |
B2T3.- ENZYMATIC STABILITY |
Introduction. Industrial importance of enzymatic stability. Factors influencing stability. Models of deactivation. Stabilization of enzymes. |
B2T4.- IMMOBILIZATION OF BIOCATALYSTS. GENERALITIES |
Immobilized biocatalyzer concept. Advantages and disadvantages of the heterogeneous catalysis versus homogeneous catalysis. Historical development. The choice of the biocatalyzer and the method. Cofactors Immobilization. Experimental determination of the enzyme activity with enzymes or immobilized cells. |
B2T5.- SYSTEMS OF IMMOBILIZATION |
Adsorption. Entrapment. Covalent bonding. New immobilization systems based on recombinant DNA technology. The use of enzymes in organic solvents and in two-phase aqueous systems. |
B2T6.- EFFECTS OF IMMOBILIZATION ON THE ACTIVITY OF THE BIOCATALYST |
Effects on the enzyme molecule. Effects of partition and dissemination. Heterogeneous Catalysis with viable cells. |
B2T7.- APPLICATIONS OF IMMOBILIZED BIOCATALYSTS |
Enzyme bioreactors. Use in the food industry. Enzymes immobilized as therapeutic agents. Analytical applications. Artificial organs. |
B2T8.- BIOSENSORS |
Concept. Functional units of a biosensor. Main fields of application. The biological reaction. Types of biosensors. |
B2T9.- DESIGN OF PROTEINS |
Introduction. Engineering versus protein design. Recognition of preserved areas and with important functional in protein families. From de protein sequence to the structure: prediction. Design of new protein structures. Site-directed mutagenesis techniques. Hybrid proteins. |
B2T10.- ENGINEERING OF PROTEINS |
The artificial evolution of proteins. Strategies. Variants of DNA shuffling. Phage and yeast display. |
B2T11.- PROTEINS OF DESIGN IN THE DEVELOPMENT OF BIOSENSORS |
Concept of generic biosensor. Modification of proteins to adapt them to its function in biosensors. |
B3T1.-PRODUCTION OF MONOCLONAL ANTIBODIES |
Fundamentals. The technique of production of monoclonal antibodies. Applications. |
B3T2.- RECOMBINANT MONOCLONAL ANTIBODIES |
Humanized monoclonal antibodies. Design monoclonal antibodies. Construction of catalytic antibodies (abzymes). |
B3T3.- STRATEGIES AND METHODS FOR OBTAINING VACCINES |
Classical systems for the production of vaccines. Antigenic determinants. Subunit vaccines. DNA vaccines. Safety of vaccines derived from biotechnology. |
B3T4.- APPLICATIONS OF THE BIOTECHNOLOGY IN THE PHARMACEUTICAL INDUSTRY |
Heterologous therapeutic proteins. Modified therapeutic proteins. Rational design of drugs. Pharmacogenomics. |
B3T5.- MOTHER CELLS |
Concept. Types. Current state of research and applications. |
B3T6.- APPLICATION OF THE BIOTECHNOLOGY IN THE FORENSIC MEDICINE |
Traces of DNA. Analysis of mini-satellite by Southern blotting. PCR-based methodologies. |
B3T7.- BIOTECHNOLOGICAL TREATMENT OF MILK WHEY |
Pollution problem and reuse of milk whey |
B3T8.- THE USE OF LIGNOCELULOSIC RESIDUES |
Importance and possibilities of use. |
B3T9.- ETHICAL AND LEGAL ASPECTS OF THE BIOTECHNOLOGY |
Safety of the biotechnology industries. The importance of the public opinion. Socio-political guidelines. Intellectual property. Patents. Modern biotechnology regulation: a historical perspective. |
Planning |
Methodologies / tests |
Competencies / Results |
Teaching hours (in-person & virtual) |
Student’s personal work hours |
Total hours |
Guest lecture / keynote speech |
A8 A12 A13 A14 A15 |
21 |
42 |
63 |
Seminar |
A14 A15 B5 B6 B8 |
4 |
12 |
16 |
Problem solving |
A15 B2 |
3 |
3 |
6 |
Objective test |
A8 A12 A13 A14 A15 B8 |
2 |
20 |
22 |
Laboratory practice |
B2 B5 B6 |
14 |
28 |
42 |
|
Personalized attention |
|
1 |
0 |
1 |
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(*)The information in the planning table is for guidance only and does not take into account the heterogeneity of the students. |
Methodologies |
Methodologies |
Description |
Guest lecture / keynote speech |
Exposición oral complementada co uso de medios audiovisuais coa finalidade de transmitir coñecementos e facilitar a aprendizaxe. |
Seminar |
Técnica de traballo en grupo que ten como finalidade a elaboración de documentos en powerpoint e word, e expostos en clase de seminarios, sobre un tema proposto polo profesor.
Os temas propostos indicaranse durante o desenvolvemento da materia.
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Problem solving |
Traballarase coa metodoloxia de aprendizaxe baseado en problemas. Plantearase un problema que pode darse na realidade e traballarase cos alumnos nas clases de grupos interactivo para intentar resolvelo.
|
Objective test |
Exame que avaliará os coñecementos teórico e prácticos adquiridos. |
Laboratory practice |
Metodoloxía que permite que os estudantes aprendan efectivamente a través da realización de actividades de carácter práctico. |
Personalized attention |
|
Description |
A atención personalizada que se describe en relación a estas metodoloxías concíbense como momentos de traballo presencial do alumno co profesor polo que implican unha participación obrigatoria para o alumno.
A forma e o momento en que se desenvolverá indicarase en relación a cada actividade ao longo do curso segundo o plan de traballo da materia.
Para o alumnado con recoñecemento de dedicación a tempo parcial e dispensa académica de exención de asistencia, o profesor adoptará as medidas que considere oportunas para non perxudicar a súa cualificación.
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Assessment |
Methodologies
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Competencies / Results |
Description
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Qualification
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Seminar |
A14 A15 B5 B6 B8 |
Avaliarase o seminario realizado polo alumno tendo en conta a capacidade para a extracción do máis relevante do total da información conseguida, a capacidade para traballar en grupo e a capacidade para expor en público.
|
15 |
Problem solving |
A15 B2 |
Traballarase coa metodoloxía de aprendizaxe baseado en problemas cos alumnos durante as clases de grupo interactivo. O alumno deberá entregar individualmente a resolución ao problema plantexado para a súa avaliación.
|
10 |
Laboratory practice |
B2 B5 B6 |
Realizarase unha proba obxectiva para avaliar os coñecementos adquiridos durante a realización das prácticas de laboratorio
|
15 |
Objective test |
A8 A12 A13 A14 A15 B8 |
Avaliarase mediante unha proba obxectiva os coñecementos adquiridos durante as clases expositivas e as clases en grupo reducido.
|
60 |
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Assessment comments |
A realización das prácticas é obrigatoria. Para poder superar a materia, a calificación tanto das prácticas como da proba obxectiva deberán ser superiores a un 40% do máximo. CONSIDERACIÓN DE ALUMNO
NON PRESENTADO (XANEIRO): Para obter a cualificación de non presentado, os
alumnos non poderán participar en máis do 20% das actividades avaliables
programadas. CONSIDERACIÓN DE ALUMNO NON PRESENTADO (XULLO): Para obter a
cualificación de non presentado bastará con non presentarse ás probas
obxectivas. Para a AVALIACIÓN NA CONVOCATORIA DE XULLO manteranse os mesmos
criterios que na convocatoria de Xaneiro: o alumno deberá entregar os boletíns
de problemas resoltos e a presentación power point resumo do seminario así como
realizar as probas obxectivas correspondentes ás sesións maxistrais e prácticas
de laboratorio. A cualificación das partes aprobadas na convocatoria de Xaneiro
manterase na de Xullo. Para o alumnado con recoñecemento de dispensa académica de exención de asistencia, o profesor adoptará as medidas que considere oportunas para non perxudicar a súa cualificación. CUALIFICACIÓN Matrícula de Honra: Distribuirase de preferencia entre os estudantes que cumpran os requisitos na avaliación de xaneiro. Para ós/ás estudantes que soliciten a CONVOCATORIA ADIANTADA DE DECEMBRO, aplicarase a normativa vixente, segundo a cal rixe a guía docente do curso en vigor. Implicacións do PLAXIO na cualificación: Aplicarase a normativa vixente, segundo a cal a realización fraudulenta das probas ou actividades de avaliación implicará directamente a cualificación de suspenso.
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Sources of information |
Basic
|
Thiel, T., Bissen, S. T., Lyons, E. M. (2001). Biotechnology: DNA to Protein. A Laboratory Project in Molecular Biology. .
Walter, J. M. y Gingold. E. B (1997). Biología Molecular y Biotecnología . Zaragoza. Acribia
Perera, J., Tormo, A., García, J. L. (2002). Ingeniería Genética. Vol I. Preparación, análisis, manipulación y clonaje del DNA. . Madrid. Síntesis
Thieman, W. J., Palladino, M. A., Thieman, W. (2004). Introduction to Biotechnology. . Benjamin Cummings, Publisher
González Siso, M. I. (1999). La Biotecnología en el tratamiento de residuos industriales . A Coruña. Universidade da Coruña. Servicio de Publicacións
Wu, W., Welsh, M. J., Kaufman, P. B., Zhang, H. H. (1997). Methods in Gene Biotechnology . CRC Press
Wink, M. (2006). An introduction to molecular Biotechnology: from molecular biological fundamentals to methods and applications in modern biotechnology. Verlag Chemie, GmbH
Ratledge, C. (2002). Basic Biotechnology. Cambridge. Cambridge University Press
Cerdán Villanueva, M. E., Freire Picos, M. A., González Siso, M. I. y Rodríguez Torres, A. M. (1997). Biología Molecular. Avances y Técnicas generales . A Coruña. Universidade da Coruña
Barnum, S.R. (2005). Biotechnology: an introduction. Belmont: Thomson
Smith, J. E. (2006). Biotecnología. Zaragoza: Acribia, D.L.
Ninfa, A. J. (2010). Fundamental laboratory approaches for biochemistry and biotechnology. Hoboken: John Wiley and Sons
Perera, J., Tormo, A., García, J. L. (2002). Ingeniería Genética. Vol II. Expresión de DNA en sistemas heterólogos.. Madrid. Síntesis
Thieman, W. J. & Palladino, M.A. (2010). Introducción a la Biotecnología. Pearson
Thieman, William J. (2009). Introduction to biotechnology. San Francisco: Pearson
Glick, B. R. (2003). Molecular Biotechnology: Principles and Application of Recombinant DNA. Washington: American Society Microbiology
Christof, M. Niemeyer y Chad A. Mirkin (2004). Nanobiotechnology: concepts, applications and perspectives. Weinheim, Wiley-VCH
Schmid, R. D. (2003). Pocket guide to biotechnology and genetic engineering . Weinheim: Wiley-VCH
Gerd Gellisen Ed. (2005). Production of recombinant proteins: novel microbial and eukaryotic expression systems. Weinheim: Wiley-VCH
Luque, J., Herráez, A. (2001). Texto Ilustrado de Biología Molecular e Ingeniería Genética . Harcourt.
Borem, A., Santos, F. R., Bowen, D. E. (2003). Understanding Biotechnology. . New Jersey: Prentice Hall PTR |
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Complementary
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Varios (2006). Guía de empresas en el sector biotecnológico español. Madrid: Genoma España |
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Recommendations |
Subjects that it is recommended to have taken before |
Biochemistry I/610G02011 | Biochemistry II/610G02012 | Biochemistry and Molecular Biology/610G02013 |
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Subjects that are recommended to be taken simultaneously |
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Subjects that continue the syllabus |
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Other comments |
Green Campus Science Faculty Program To contribute to achieving an immediate sustainable environment and comply with point 6 of the "Environmental Declaration of the Faculty of Sciences (2020)", the documentary work carried out in this area: a. They will be requested mainly in virtual format and computer support. B. To do on paper: - Plastics will not be used. - Double-sided prints will be made. - Recycled paper will be used. - Drafts will be avoided. |
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