Máster Universitario en Bioloxía Molecular, Celular e Xenética (semipresencial)
Descriptors
Cycle
Period
Year
Type
Credits
Official Master's Degree
2nd four-month period
First
Optional
3
Language
Spanish
Galician
English
Teaching method
Hybrid
Prerequisites
Department
Bioloxía Departamento profesorado máster
Coordinador
Vila Taboada, Marta
E-mail
marta.vila.taboada@udc.es
Lecturers
Becerra Fernandez, Manuel
Vila Taboada, Marta
E-mail
manuel.becerra@udc.es
marta.vila.taboada@udc.es
Web
General description
Denomínase xenómica ao estudo integral do funcionamento, evolución e orixe dos xenomas. A xenómica utiliza coñecementos derivados de distintas disciplinas como xenética, bioloxía molecular, bioquímica, informática, estatística, matemáticas e física.
A diferenza da xenética clásica que a partires dun fenotipo (xeralmente mutante) procura o xene ou xenes responsables dese fenotipo, a xenómica ten como obxectivo predicir a función dos xenes a partir da súa secuencia ou das súas interaccións con outros xenes.
As denominadas ciencias ómicas están na vangarda da ciencia, feito debido ás posibilidades abertas polas novas tecnoloxías de secuenciación masiva, aos avances en bioinformática e aos algoritmos cada vez máis sofisticados para análise de xenomas completos.
Study programme competencies
Code
Study programme competences / results
A3
Skills of understanding the functioning of cells through the structural organization, biochemistry, gene expression and genetic variability.
A11
Skills of understanding the structure, dynamics and evolution of genomes and to apply tools necessary to his study.
B1
Analysis skills to understand biological problems in connection with the Molecular and Cellular Biology and Genetics.
B5
Ability to draft, represent, analyze, interpret and present technical documentation and relevant data in the field of the branch of knowledge of the master's degree in the native language and at least in another International diffusion language.
B9
Skills of preparation, show and defense of a work.
C2
Ability to know and use appropriately the technical terminology of the field of knowledge of the master, in the native language and in English, as a language of international diffusion in this field
C3
Using ICT in working contexts and lifelong learning.
C8
Valuing the importance of research, innovation and technological development for the socioeconomic and cultural progress of society.
Learning aims
Learning outcomes
Study programme competences / results
To learn the basics of the different molecular techniques used in genomics, with particular emphasis in NGS
AR3 AR11
CC3
To acquire an updated view about the current scope and future perspectives of structural, functional and evolutionary genomics
AR3 AR11
BR1 BR5 BR9
CC2 CC8
To understand how genomes evolve and how molecular and bioinformatic tools are used for that purpose
AR3 AR11
BR1 BR5 BR9
CC2 CC8
DNA microarrays: experimental set up and data analysis.
AR3 AR11
Contents
Topic
Sub-topic
Whole Genome Sequencing
Annotation
Comparative genomics
Next Generation Sequencing (NGS)
Platforms
Paired-end libraries
Introduction to data analysis
Metagenomics
Metabarcoding
Clinical genomics
Amplicon-seq
Panel-seq
Exome-seq
Pharmacogenomics
Single Nucleotide Polymorphisms (SNPs)
Genome wide association studies (GWAS)
Digital genetic testing
Functional genomics
Transcriptome analysis: microarrays and NGS (RNA-seq)
Computer lab
1. Using GALAXY for analysis of NGS data.
2. Gene expression analysis using GALAXY.
3. Pharmacogenomic analysis using PHARMKGB.
4. Introduction to the Intregative Genomics Viewer (IGV).
Planning
Methodologies / tests
Competencies / Results
Teaching hours (in-person & virtual)
Student’s personal work hours
Total hours
ICT practicals
A3 A11 B1 B5 B9 C2 C3
3
24
27
Guest lecture / keynote speech
A3 A11 B1 C8
2
40
42
Objective test
A3 A11 B1 C8
2
0
2
Personalized attention
4
0
4
(*)The information in the planning table is for guidance only and does not take into account the heterogeneity of the students.
Methodologies
Methodologies
Description
ICT practicals
The students work on their own web-based investigations and present their results to each other (active learnning).
Guest lecture / keynote speech
The instructors explain the main contents of each topic interacting as much as possible with the students. The student will be able to attend the face-to-face sessions synchronously through TEAMS. Sessions will be recorded for viewing asynchronously.
Objective test
Test conducted through the Moodle platform to evaluate the knowledge acquired.
Personalized attention
Methodologies
ICT practicals
Description
Instructors will typically be available via email/MS TEAMS. Students can arrange for in-person tutoring sessions.
Assessment
Methodologies
Competencies / Results
Description
Qualification
Objective test
A3 A11 B1 C8
In order to pass the subject, all students will have to take a multiple choice test and/or short-answer questionnaire.
70
ICT practicals
A3 A11 B1 B5 B9 C2 C3
All students will have to submit two reports following the guidelines provided by each instructor. In these reports, students will answer questions and/or solve exercises using their own computer and the software introduced during the computer labs.
30
Assessment comments
Students scoring at least 50 (out of 100) points but not reaching the aforementioned thresholds (ICT practicals: 15 out of 30 points; Objective test; 28 out of 70 points) will be awarded a 4.5 (out of 10) score. When resitting, they can choose to take both exams or only the failed one. Mark "A with distinction" will only be awarded to outstanding students passing the subject in May. Students will be scored as "ABSENT" (Non presentado) only when not involved in any of the assessed activities. In the case of exceptional circumstances, lecturers may assist the student to improve his/her learning process and/or catch up on missed work/assessments. The student is responsible for liaising with his/her lecturer to organise this assistance by e.g. applying for: an extended deadline to present his/her work or taking an exam in a different date. The coordinator can request evidence about the reason for such an application. Implications of PLAGIARISM in the qualification: the current UDC regulations will be applied.
Sources of information
Basic
McLachlan, G. J., Do, K-A., Ambroise, C (2004). Analyzing Microarray Gene Expression Data. Wiley-Interscience. John Wiley & Sons
Pevsner, J. (2015). Bioinformatics and Functional Genomics. Wiley Blackwell
Kulkarni, S., Pfeifer, J. (2015). Clinical Genomics. A guide to Clinical NGS. Academic Press, Elsevier
Robison, P.N., Piro, R.M., Jäger, M. (2018). Computational Exome and Genome Analysis. CRC Press, Taylor & Francis Group
Bowtell, D., Sambrook, J. (2003). DNA Microarrays. Cold Spring Harbor Laboratory Press.
Allison, David B., et al (2006). DNA microarrays and related genomics techniques design, analysis, and interpretation of experiments. Chapman & Hall/CRC
E. Rinaldis, A. Lahm. (2007). DNA microarrays: current applications. Wymondham: Horizon Bioscience
Brown, T. A. (2018). Genomes4. Garland Science
Complementary
(). .
Zhanjiang, Liu (2007). Aquaculture genome techonologies. Blackwell
Dale Jeremy (2008). From genes to genomes: concepst and applications of DNA technology. John Wiley & Sons
Sensen, Christoph W. (2005). Handbook of genome research genomics, proteomics, metabolism, bioinformatics, ethical & legal issues . Wiley-VCH
RECURSOS EN INTERNET:
Biological database compilation at NAR: http://nar.oupjournals.org/content/vol29/issue1
DOE Joint Genome Institut. Why sequence them? http://www.jgi.doe.gov/sequencing/why/index.html
Subjects that it is recommended to have taken before
Cellular Techniques/610441001
Molecular Techniques/610441002
Genetic Variation Mechanisms/610441005
Regulation of gene expression/610441006
Bioinformatics and Biomolecular models /610441021
Subjects that are recommended to be taken simultaneously
Proteomics/610441014
Chromosomes. structure. function and evolution /610441016
Human Genetics/610441017
Genetic Toxicology /610441018
Subjects that continue the syllabus
Project/610441023
Other comments
Do not take this subject if your level of English is lower than B1. Green Campus Faculty of Sciences Program: to help achieve a sustainable immediate environment and comply with point 6 of the "Environmental Declaration of the Faculty of Sciences (2020)", the documentary works to be carried out in this subject will be requested in virtual format and computer support.
(*)The teaching guide is the document in which the URV publishes the information about all its courses. It is a public document and cannot be modified. Only in exceptional cases can it be revised by the competent agent or duly revised so that it is in line with current legislation.