The passing grade will be obtained for a final grade of 5 out of 10. The final grade, both of first and second opportunity, will be based on the evaluation of the following aspects:

• 40% of the final mark: continuous evaluation based on the following contributions:

Evaluation of problems submitted for each topic: 10%.

Evaluation of practical cases: 20%

Oral presentation of a research article: 10%.

• 60% of the final mark: evaluation of the final exam of the subject with conceptual questions and problems, complementary to the continuous evaluation both in the first and second opportunity and in any of the scenarios. It will be necessary to obtain a minimum mark of 4 out of 10 in the exam to pass the course.

The assessment of students who repeat the subject will be governed by the same assessment standards as that of students taking the subject for the first time.

PLAGIARISM AND MISUSE OF TECHNOLOGIES IN THE CONDUCT OF TASKS OR TESTS: "For cases of fraudulent execution of exercises or tests, the provisions of the Regulations for the evaluation of student academic performance and revision of qualifications will apply."

CONTINGENCY PLAN FOR REMOTE TEACHING ACTIVITIES: The evaluation system will be the same regardless of the type of teaching used (face-to-face or virtual), with the only difference that the evaluation activities will be carried out, according to what the competent authorities establish, either in person in the classroom or remotely through the telematic means available at the USC.

Learning outcomes	Study programme competences
	AC1 AC3 AC7	BC2 BC3 BC7 BC10 BC11	CC1 CC3 CC4
	AC1 AC3 AC7	BC2 BC3 BC7 BC10 BC11	CC1 CC3 CC4
	AC1 AC3 AC7	BC2 BC3 BC7 BC10 BC11	CC1 CC3 CC4
	AC1 AC3 AC7	BC2 BC3 BC7 BC10 BC11	CC1 CC3 CC4
	AC1 AC3 AC7	BC2 BC3 BC7 BC10 BC11	CC1 CC3 CC4
	AC1 AC3 AC7	BC2 BC3 BC7 BC10 BC11	CC1 CC3 CC4
	AC1 AC3 AC7	BC2 BC3 BC7 BC10 BC11	CC1 CC3 CC4

Topic	Sub-topic
1. Fundamentals of electronic spectroscopy and fluorescence spectroscopy	Luminiscent phenomena. Radiative and nonradiative processes. Fluorescence excitation and emission spectra. Fluorescence quantum yield. Fluorescence lifetime. Effect of environment on fluorescence.
2. Experimental techniques	Measurement of fluorescence spectra: the spectrofluorometer. Correction of excitation and emission spectra. Measurement of fluorescence lifetimes. Measurement of fluorescence polarization. Ultrafast techniques. Single-molecule fluorescence. Fluorescence Microscopy.
3. Fluorescence quenching	Collisional or dynamic quenching. Stern-Volmer equation. Static quenching. Static and dynamic quenching. Applications to study complex formation and microheterogeneous systems.
4. Excited electronic states and photochemistry	Excited-state complex formation: excimers and exciplexes. Photoinduced electron transfer. Photoinduced proton transfer. Other photochemical reactions.
5. Electronic energy transfer	Electronic energy-transfer mechanisms. Förster Resonance Energy Transfer (FRET). Applications for the measurement of molecular distances and the study of supramolecular associations. Dexter mechanism of energy transfer: photosensitization and photodynamic therapy.
6. Fluorescence probes	Classes of fluorescence probes: intrinsic and extrinsic. Green Fluorescence Protein. Quantum dots. Applications in biomedicine, analyses, environment, and materials studies.

Methodologies / tests	Competencies	Ordinary class hours	Student’s personal work hours	Total hours
Guest lecture / keynote speech	A1 B2 B3 B10	12	6	18
Seminar	A7 B2 B3 B7 B10	7	13	20
Supervised projects	A3 B2 B3 B7 B10 B11 C1 C3 C4	20	13	33
Speaking test	C1 C3	0	0	0
Objective test	A1 A3 A7 B2 B10 C4	2	0	2

Personalized attention		2	0	2

(*)The information in the planning table is for guidance only and does not take into account the heterogeneity of the students.

Methodologies	Description
Guest lecture / keynote speech
Seminar
Supervised projects
Speaking test
Objective test

Methodologies	Competencies	Description	Qualification
Seminar	A7 B2 B3 B7 B10	Evaluation of problems submitted for each topic: 10%. Evaluation of practical cases: 20%	30
Supervised projects	A3 B2 B3 B7 B10 B11 C1 C3 C4	Oral presentation of a research article: 10%.	10
Objective test	A1 A3 A7 B2 B10 C4	60% of the final mark: evaluation of the final exam of the subject with conceptual questions and problems	60

Assessment comments
The passing grade will be obtained for a final grade of 5 out of 10. The final grade, both of first and second opportunity, will be based on the evaluation of the following aspects: • 40% of the final mark: continuous evaluation based on the following contributions: Evaluation of problems submitted for each topic: 10%. Evaluation of practical cases: 20% Oral presentation of a research article: 10%. • 60% of the final mark: evaluation of the final exam of the subject with conceptual questions and problems, complementary to the continuous evaluation both in the first and second opportunity and in any of the scenarios. It will be necessary to obtain a minimum mark of 4 out of 10 in the exam to pass the course. The assessment of students who repeat the subject will be governed by the same assessment standards as that of students taking the subject for the first time. PLAGIARISM AND MISUSE OF TECHNOLOGIES IN THE CONDUCT OF TASKS OR TESTS: "For cases of fraudulent execution of exercises or tests, the provisions of the Regulations for the evaluation of student academic performance and revision of qualifications will apply." CONTINGENCY PLAN FOR REMOTE TEACHING ACTIVITIES: The evaluation system will be the same regardless of the type of teaching used (face-to-face or virtual), with the only difference that the evaluation activities will be carried out, according to what the competent authorities establish, either in person in the classroom or remotely through the telematic means available at the USC.

Basic	Bernard Valeur (2012). Molecular Fluorescence. Principles and Applications, 2nd Ed. Wiley-VCH, Weinheim Petr Klán y Jacob Wirz (2009). Photochemistry of Organic Compounds: From Concepts to Practice,. Wiley, Chichester Joseph R. Lakowicz (2006). Principles of Fluorescence Spectroscopy, 3rd Ed. Springer, New York Paul R. Selvin y Taekjip Ha (2008). Single-Molecule Techniques. A laboratory manual. Cold Spring Harbor Laboratory Press, New York
	Review and research articles related to the subject.
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