Materia obrigatoria centrada nos cultivos celulares animais e vexetais, así como nos fundamentos e aplicacións de técnicas de microscopía, análise de imaxe, citometría de fluxo e análise de cromosomas.
Pendente de incluir polos servicios de GADU, a profesora do INIBIC Dra. Dna. María José López Armada (MA.Jose.Lopez.Armada@sergas.es).
Study programme competencies
Code
Study programme competences
A1
Skills of using usual techniques and instruments in the cellular, biological and molecular research: that are able to use techniques and instruments as well as understanding potentials of their uses and applications.
A2
Skills of working in a sure way in the laboratories knowing operation handbooks and actions to avoid incidents of risk.
A13
Skills to become a professional in health, pharmacy, veterinary, animal production, biotechnology or food sectors.
B3
Skills of management of the information: that are able to gather and to understand relevant information and results, obtaining conclusions and to prepare reasoned reports on scientific and biotechnological questions
B4
Organization and work planning skills: that are able to manage the use of the time as well as available resources and to organize the work in the laboratory.
Learning aims
Learning outcomes
Study programme competences
To understand the theoretical foundations on which microscopy (including image analysis), (plant and animal) cell culture, flow cytometry and cytogenetic techniques are based.
AR1 AR2
To acquire basic skills in the management and use of instrumental and units required for the development of cellular techniques.
AR1 AR2 AR13
To know the applications of the different cellular techniques.
AR1
To design, plan and conduct experiments regarding the techniques learned.
AR1 AR2
BR3 BR4
Contents
Topic
Sub-topic
Microscopy and image analysis
Fundamentals, techniques and applications of light and electron microscopy. Fluorescence and confocal scanning microscopy: advanced techniques and applications. Introduction to image processing and image analysis
(Animal & Plant) cell cultures
Introduction to cell cultures. Types of cell cultures. Cell culture requirements. Quantification of cellular parameters. Contamination. Cytotoxicity.
In vitro cultures of plant tissues. Callus. Cultivation of plant cells in suspension.
Flow cytometry
General principles and methods of cytometry. Sample preparation and standardization of analysis procedures. Functional analysis of cells.
Cytogenetic techniques
Chromosome preparation and karyotype. Conventional in situ hybridization. Advanced techniques of fluorescence in situ hybridization (FISH).
Planning
Methodologies / tests
Competencies
Ordinary class hours
Student’s personal work hours
Total hours
Guest lecture / keynote speech
A1
14
28
42
Laboratory practice
A1 A2 A13 B3 B4
28
42
70
Supervised projects
A1 B3
0
19
19
Mixed objective/subjective test
A1 B3
2
15
17
Personalized attention
2
0
2
(*)The information in the planning table is for guidance only and does not take into account the heterogeneity of the students.
Methodologies
Methodologies
Description
Guest lecture / keynote speech
Teacher will present the theoretical and practical contents of the subject (of the different techniques currently used in Cell Biology).
Laboratory practice
Practical sessions represent an indispensable part of the course, in which practical aspects and applications of the different cellular techniques will be approached. Practical sessions will be developed in concrete laboratories and places: laboratories of the Faculty of Sciences, Laboratories of Biomedical Research Institute (INIBIC), Scientific Research Support Services (SAI) of UDC.
Students will develop laboratory protocols and attend demonstrations about the use of research units.
Supervised projects
Students must carry out works, resolve problems and/or questions about specific aspects of the techniques used.
Mixed objective/subjective test
It will consist of a written exam with questions-test and/or short answer questions about theoretical and practical contents and applications of the cellular techniques.
Personalized attention
Methodologies
Supervised projects
Description
Students (individually or in small groups) may consult their doubts about the contents and activities of the subject via phone and/or electronic support.
Assessment
Methodologies
Competencies
Description
Qualification
Mixed objective/subjective test
A1 B3
The acquisition of knowledge about the theoretical foundations and applications, clarity of explanations, ability to integrate and link information handled and the ability to interpret data and solve problems will be taken into account.
50
Laboratory practice
A1 A2 A13 B3 B4
Attendance, skill in scheduled tasks and knowledge of the potential risks in laboratory practices will be assessed.
20
Supervised projects
A1 B3
The ability to design (and plan) experiments, interpretate data and solve problems will be assessed.
30
Assessment comments
In order to be evaluated, students must attend to practical sessions.
In july there is the opportunity to retake only the tests. The january's score of supervised projects and practices are maintained.
Preferably, first class honors will be awarded in january.
Full-time and part-time students will be evaluated following this qualification guideline.
Sources of information
Basic
CELL CULTURE
Basra, A.S. (2000). Plant growth regulators in agriculture and horticulture. Their role and commercial uses. Ed. Food Products Press.
Benítez Burraco, A. (2005). Avances recientes en Biotecnología vegetal e ingeniería genética de plantas. Editorial Reverté.
Boulton, A.A. e col. (1992). Practical cell culture techniques. Humana Press.
Butler, M. (2008). Animal cell culture and technology. Taylor & Francis, 2nd edition.
Collin, H.A. e Edwars, S. (1998). Plant cell culture. Guilford Bios Scientific Publishers.
Doyle, A. e Griffiths, J.B. (2000). Cell and tissue culture for medical research. John Wiley and Sons.
Fedoroff, S. e Richardson, A. (1992). Protocols for neural cell culture. Humana Press.
Freshney, R.I. (2010). Culture of animal cells. A manual of basic technique and specialized applications. Wiley-Liss, Inc. 6ª Edition.
Hammond, J., McGarvey, P., Yusibov, V. (1999). Plant Biotechnology. New products and Applications. Springer verlag.
Loyola-Vargas, V.M. e Vázquez-Flota F. (2006). Plant cell culture protocols. Humana Press. 2nd Edition.
Pollard, J.W. e Walker, J.M. (1997). Basic cell culture protocols. Humana Press.
Shaw, A.J. (1996). Epithelial cell culture. A practical approach. Oxford University Press.
Taji, A., Kumar, P., Lakshmanan, P. (2002). In vitro plant breeeding. Food Products Press.
Trigiano, R.N. e Gray, D.J. (2004). Plant development and biotechnology. CRC Press.
Tzfira, T. e Citovsky, V. (2006). Agrobacterium-mediated genetic transformation of plants: biology and biotechnology. Curr. Opin. Biotechnol. 17:147–154.
Vunjak-Novakovic, G. & Freshney, R.I. (2006). Culture of cells for tissue engineering. Wiley-Liss, Inc.
MICROSCOPIC TECHNIQUES AND IMAGE ANALYSIS
Watt, Ian M. 1996. The principles and practice of electron microscopy. Cambridge University Press
Hoppert, M. 1998. Electron microscopy in microbiology. Bios Scientific Publishers
Bozzola, John J. 1999.Electron microscopy : principles and techniques for biologists. Jones and Bartlett Publishers.
Dykstra, Michael J. 2003. Biological electron microscopy theory, techniques, and troubleshooting. Kluwer Academic/Plenum Publishers
Robin Harris. 1991. Electron microscopy in biology a practical approach. Oxford University Press.
Hunter, Elaine Evelyn. 1984. Practical electron microscopy a beginner's illustrated guide. Praeger, cop.
Slayter, Elizabeth M. 2000. Light and electron microscopy. Cambridge University Press
Herman, B. 1998. Fluorescence microscopy. Bios Scientific Publishers
Donat-P. Häder. 1992. Image analysis in biology. CRC Press, cop.
Pertusa, JF. Técnicas de Análisis de imagen. Aplicaciones en Biología. Publicaciones de la Universidad de Valencia. 2003
FLOW CYTOMETRY
Ormerod, M.G. (2009). Flow Cytometry: A Basic Introduction. 2a Ed. IRL Practical Approach series. Oxford University Press.
Subjects that it is recommended to have taken before
Subjects that are recommended to be taken simultaneously
Subjects that continue the syllabus
Other comments
(*)The teaching guide is the document in which the URV publishes the information about all its courses. It is a public document and cannot be modified. Only in exceptional cases can it be revised by the competent agent or duly revised so that it is in line with current legislation.