Identifying Data 2019/20
Subject (*) Cellular Techniques Code 610441001
Study programme
Mestrado Universitario en Bioloxía Molecular , Celular e Xenética
Descriptors Cycle Period Year Type Credits
Official Master's Degree 1st four-month period
First Obligatory 6
Language
Spanish
Galician
English
Teaching method Face-to-face
Prerequisites
Department Bioloxía
Departamento profesorado máster
Coordinador
Yañez Sanchez, Julian
E-mail
julian.yanez@udc.es
Lecturers
Bernal Pita da Veiga, angeles
Castro Castro, Antonio Manuel
Folgueira Otero, Mónica
Insua Pombo, Ana Maria
López Armada, María José
Rioboo Blanco, Carmen
Yañez Sanchez, Julian
E-mail
angeles.bernal@udc.es
antonio.castro@udc.es
m.folgueira@udc.es
ana.insua@udc.es
carmen.rioboo@udc.es
julian.yanez@udc.es
Web http://https://campusvirtual.udc.es/moodle/
General description Materia obrigatoria centrada nos cultivos celulares animais e vexetais, así como nos fundamentos e aplicacións de técnicas de microscopía, análise de imaxe, citometría de fluxo e análise de cromosomas.

Pendente de incluir polos servicios de GADU, a profesora do INIBIC Dra. Dna. María José López Armada (MA.Jose.Lopez.Armada@sergas.es).
Contingency plan

Study programme competencies
Code Study programme competences
A1 Skills of using usual techniques and instruments in the cellular, biological and molecular research: that are able to use techniques and instruments as well as understanding potentials of their uses and applications.
A2 Skills of working in a sure way in the laboratories knowing operation handbooks and actions to avoid incidents of risk.
A13 Skills to become a professional in health, pharmacy, veterinary, animal production, biotechnology or food sectors.
B3 Skills of management of the information: that are able to gather and to understand relevant information and results, obtaining conclusions and to prepare reasoned reports on scientific and biotechnological questions
B4 Organization and work planning skills: that are able to manage the use of the time as well as available resources and to organize the work in the laboratory.

Learning aims
Learning outcomes Study programme competences
To understand the theoretical foundations on which microscopy (including image analysis), (plant and animal) cell culture, flow cytometry and cytogenetic techniques are based. AR1
AR2
To acquire basic skills in the management and use of instrumental and units required for the development of cellular techniques. AR1
AR2
AR13
To know the applications of the different cellular techniques. AR1
To design, plan and conduct experiments regarding the techniques learned. AR1
AR2
BR3
BR4

Contents
Topic Sub-topic
Microscopy and image analysis Fundamentals, techniques and applications of light and electron microscopy. Fluorescence and confocal scanning microscopy: advanced techniques and applications. Introduction to image processing and image analysis
(Animal & Plant) cell cultures Introduction to cell cultures. Types of cell cultures. Cell culture requirements. Quantification of cellular parameters. Contamination. Cytotoxicity.
In vitro cultures of plant tissues. Callus. Cultivation of plant cells in suspension.
Flow cytometry General principles and methods of cytometry. Sample preparation and standardization of analysis procedures. Functional analysis of cells.
Cytogenetic techniques Chromosome preparation and karyotype. Conventional in situ hybridization. Advanced techniques of fluorescence in situ hybridization (FISH).

Planning
Methodologies / tests Competencies Ordinary class hours Student’s personal work hours Total hours
Guest lecture / keynote speech A1 14 28 42
Laboratory practice A1 A2 A13 B3 B4 28 42 70
Supervised projects A1 B3 0 19 19
Mixed objective/subjective test A1 B3 2 15 17
 
Personalized attention 2 0 2
 
(*)The information in the planning table is for guidance only and does not take into account the heterogeneity of the students.

Methodologies
Methodologies Description
Guest lecture / keynote speech Teacher will present the theoretical and practical contents of the subject (of the different techniques currently used in Cell Biology).
Laboratory practice Practical sessions represent an indispensable part of the course, in which practical aspects and applications of the different cellular techniques will be approached. Practical sessions will be developed in concrete laboratories and places: laboratories of the Faculty of Sciences, Laboratories of Biomedical Research Institute (INIBIC), Scientific Research Support Services (SAI) of UDC.
Students will develop laboratory protocols and attend demonstrations about the use of research units.
Supervised projects Students must carry out works, resolve problems and/or questions about specific aspects of the techniques used.
Mixed objective/subjective test It will consist of a written exam with questions-test and/or short answer questions about theoretical and practical contents and applications of the cellular techniques.

Personalized attention
Methodologies
Supervised projects
Description
Students (individually or in small groups) may consult their doubts about the contents and activities of the subject via phone and/or electronic support.

Assessment
Methodologies Competencies Description Qualification
Mixed objective/subjective test A1 B3 The acquisition of knowledge about the theoretical foundations and applications, clarity of explanations, ability to integrate and link information handled and the ability to interpret data and solve problems will be taken into account. 50
Laboratory practice A1 A2 A13 B3 B4 Attendance, skill in scheduled tasks and knowledge of the potential risks in laboratory practices will be assessed. 20
Supervised projects A1 B3 The ability to design (and plan) experiments, interpretate data and solve problems will be assessed. 30
 
Assessment comments

In order to be evaluated, students must attend to practical sessions.

In july there is the opportunity to retake only the tests. The january's score of supervised projects and practices are maintained.

Preferably, first class honors will be awarded in january.

Full-time and part-time students will be evaluated following this qualification guideline.


Sources of information
Basic

CELL CULTURE

Basra, A.S. (2000). Plant growth regulators in agriculture and horticulture. Their role and commercial uses. Ed. Food Products Press.

Benítez Burraco, A. (2005). Avances recientes en Biotecnología vegetal e ingeniería genética de plantas. Editorial Reverté.

Boulton, A.A. e col. (1992). Practical cell culture techniques. Humana Press.

Butler, M. (2008). Animal cell culture and technology. Taylor & Francis, 2nd edition.

Collin, H.A. e Edwars, S. (1998). Plant cell culture. Guilford Bios Scientific Publishers.

Davis, J.M. (2011). Animal cell culture. Essential methods. Wiley-Blackwell.

Doyle, A. e Griffiths, J.B. (2000). Cell and tissue culture for medical research. John Wiley and Sons.

Fedoroff, S. e Richardson, A. (1992). Protocols for neural cell culture. Humana Press.

Freshney, R.I. (2010). Culture of animal cells. A manual of basic technique and specialized applications. Wiley-Liss, Inc. 6ª Edition.

Hammond, J., McGarvey, P., Yusibov, V. (1999). Plant Biotechnology. New products and Applications. Springer verlag.

Loyola-Vargas, V.M. e Vázquez-Flota F. (2006). Plant cell culture protocols. Humana Press. 2nd Edition.

Pollard, J.W. e Walker, J.M. (1997). Basic cell culture protocols. Humana Press.

Shaw, A.J. (1996). Epithelial cell culture. A practical approach. Oxford University Press.

Taji, A., Kumar, P., Lakshmanan, P. (2002). In vitro plant breeeding. Food Products Press.

Trigiano, R.N. e Gray, D.J. (2004). Plant development and biotechnology. CRC Press.

Tzfira, T. e Citovsky, V. (2006). Agrobacterium-mediated genetic transformation of plants: biology and biotechnology. Curr. Opin. Biotechnol. 17:147–154.

Vunjak-Novakovic, G. & Freshney, R.I. (2006). Culture of cells for tissue engineering. Wiley-Liss, Inc.

MICROSCOPIC TECHNIQUES AND IMAGE ANALYSIS

Watt, Ian M. 1996. The principles and practice of electron microscopy. Cambridge University Press

Hoppert, M. 1998. Electron microscopy in microbiology. Bios Scientific Publishers

Bozzola, John J. 1999.Electron microscopy : principles and techniques for biologists. Jones and Bartlett Publishers.

Dykstra, Michael J. 2003. Biological electron microscopy theory, techniques, and troubleshooting. Kluwer Academic/Plenum Publishers

Robin Harris. 1991. Electron microscopy in biology a practical approach.  Oxford University Press.

Hunter, Elaine Evelyn. 1984. Practical electron microscopy a beginner's illustrated guide. Praeger, cop.

Slayter, Elizabeth M. 2000.  Light and electron microscopy. Cambridge University Press

Herman, B. 1998. Fluorescence microscopy. Bios Scientific Publishers

Donat-P. Häder. 1992. Image analysis in biology. CRC Press, cop.

Pertusa, JF. Técnicas de Análisis de imagen. Aplicaciones en Biología. Publicaciones de la Universidad de Valencia. 2003

FLOW CYTOMETRY

Ormerod, M.G. (2009). Flow Cytometry: A Basic Introduction. 2a Ed. IRL Practical Approach series. Oxford University Press.

Shapiro, H.M. (2004). Practical flow cytometry. Wiley-Liss. 4a ed. New York.

CYTOGENETIC TECHNIQUES

Czepulkowski, B. (2001) Analyzing chromosomes. BIOS Scientific Publishers, Oxford.

Gosden, J.R. (1994). Chromosome analysis protocols. Humana Press, Totowa (New Jersey).

Liehr, T. (2006). Multicolor FISH in human cytogenetics. Karger, Basel.

Liehr, T. (2009). Fluorescence in situ hybridization (FISH)-application guide. Springer-Verlag, Berlin.

Leitch, A.R., Schwarzacher, T., Jackson, D. (1994). In situ hybridization: a practical guide. Bios Scientific Publishers, Oxford.

Verma, R.S. e Babu, A. (1989). Human chromosomes: manual of basic techniques. Pergamon Press, New York.

Complementary

- For scientific articles see Moodle.


- Web


General
PubMed: http://www.ncbi.nlm.nih.gov/sites/pubmed


Cell culture
-http://www.cultek.com/aplicaciones.asp?P=Aplicacion_Cultivos_Celulares&opc=introduccion


Flow cytometry
-Cytometry: http:www3.interscience.wiley.com/cgi-bin/jhome/33945


Microscopic techniques and image analysis
http://zeiss-campus.magnet.fsu.edu/index.html
http://www.microscopyu.com/tutorials/
http://www.olympusfluoview.com/index.html
http://w3.uniroma1.it/MEDICFISIO/microscopy.htm
http://rsbweb.nih.gov/ij/index.html
http://www.invitrogen.com/site/us/en/home/support/Research-Tools/Fluorescence-SpectraViewer.html


Recommendations
Subjects that it is recommended to have taken before

Subjects that are recommended to be taken simultaneously

Subjects that continue the syllabus

Other comments


(*)The teaching guide is the document in which the URV publishes the information about all its courses. It is a public document and cannot be modified. Only in exceptional cases can it be revised by the competent agent or duly revised so that it is in line with current legislation.