| Study programme competencies |
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Code
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Study programme competences
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| A1 |
Skills of using usual techniques and instruments in the cellular, biological and molecular research: that are able to use techniques and instruments as well as understanding potentials of their uses and applications. |
| A2 |
Skills of working in a sure way in the laboratories knowing operation handbooks and actions to avoid incidents of risk. |
| A3 |
Skills of understanding the functioning of cells through the structural organization, biochemistry, gene expression and genetic variability. |
| A5 |
Skills of understanding the microorganisms' role as pathogenic agents and as biotechnological tools. |
| A6 |
Skills of understanding the functioning of cells through the structural organization, biochemistry, gene expression and genetic variability. |
| A9 |
Skills of understanding the structure and dynamics of proteins to individual and proteomic level, as well as the techniques that are necessary to analyze them and to study their interactions with other biomolecules. |
| A10 |
Skills of modifying genes, proteins and chromosomes with biotechnological applications |
| A11 |
Skills of understanding the structure, dynamics and evolution of genomes and to apply tools necessary to his study. |
| A13 |
Skills to become a professional in health, pharmacy, veterinary, animal production, biotechnology or food sectors. |
| B1 |
Analysis skills to understand biological problems in connection with the Molecular and Cellular Biology and Genetics. |
| B2 |
Skills of decision making for the problem solving: that are able to apply theoretical knowledges and practical acquired in the formulation of biological problems and the looking for solutions. |
| B3 |
Skills of management of the information: that are able to gather and to understand relevant information and results, obtaining conclusions and to prepare reasoned reports on scientific and biotechnological questions |
| B5 |
Correct oral and written communication on scientific topics in the native language and at least in another International diffusion language. |
| B6 |
Skills of team work: that are able to keep efficient interpersonal relationships in an interdisciplinary and international work context, with respect for the cultural diversity. |
| B9 |
Skills of preparation, show and defense of a work. |
| C3 |
Using ICT in working contexts and lifelong learning. |
| C8 |
Valuing the importance of research, innovation and technological development for the socioeconomic and cultural progress of society. |
| Learning aims |
| Learning outcomes |
Study programme competences |
| Skills of make presentations regarding the actual knowledge state in the field.
Skills of understanding the functioning of cells through the structural organization, biochemistry, gene expression and genetic variability
Analysis skills to understand biological problems in connection with the Molecular and Cellular Biology and Genetics
Skills of understanding the functioning of cells through the structural organization, biochemistry, gene expression and genetic variability
Skills of decision making for the problem solving: that are able to apply theoretical knowledges and practical acquired in the formulation of biological problems and the looking for solutions.
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AR1
AR2
AR3
AR5
AR6
AR9
AR10
AR11
AR13
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BR1
BR2
BR3
BR5
BR6
BR9
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CC3
CC8
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| .-Skills to be critical with the results and the hypothesis as well as evaluation and interpretation of results . |
AR13
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BR1
BR2
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CC8
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| Contents |
| Topic |
Sub-topic |
| Topic 1 |
Introduction to techniques and methodology to study the regulation of gene expression. |
| Topic 2 |
The transcriptional machinery in eukaryotes. Transcripcional general factors (TFII) and TAFs. The mediator complex and the complex SRB10 kinase. |
| Topic 3 |
The complexes that remodel chromatin. ATP-hydrolyzing complexes. SWI/SNF and ISWI complexes. |
| Topic 4 |
SAGA complex and counterparts. Acetylation and regulation of gene expression: HATs. The gene repression processes and deacetylation. The repression mechanisms of gene methylation. |
| Topic 5 |
Specific transcripcion factors. The signaling cascades and specific transcription factors. Nuclear receptors and transcriptional control. |
| Topic 6 |
New concepts in the regulation of gene expression. Transcripcion factories and other models. |
| Topic 7 |
RNA processing and nucleous-cytoplasm transport: the machinery of RNA cleavaje and polyadenylation, transport across the Nuclear Pore Complex and factors involved. Cytosolic polyadenylation. |
| Topic 8 |
RNA secondary structures and protein-factors with RNA-binding domains in the regulation of mRNA levels. mRNA stability.
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| Topic 9 |
RNA and protein translation. Local protein translation. The 3´-UTRs in the translation efficiency process. RNA Editing. |
| Topic 10 |
micro and siRNAs in the regulation of Gene Expression: basic and applied aspects. |
| Planning |
| Methodologies / tests |
Competencies |
Ordinary class hours |
Student’s personal work hours |
Total hours |
| Seminar |
A5 A6 A9 A10 A11 B3 B5 B6 B9 C3 C8 |
2 |
8 |
10 |
| Laboratory practice |
A1 A2 A3 B1 B2 |
7 |
7 |
14 |
| Guest lecture / keynote speech |
A5 A6 A9 A10 A11 |
8 |
16 |
24 |
| Problem solving |
A13 B1 B2 |
2 |
8 |
10 |
| Objective test |
A5 A6 A9 A10 A11 A13 |
2 |
14 |
16 |
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| Personalized attention |
|
1 |
0 |
1 |
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| (*)The information in the planning table is for guidance only and does not take into account the heterogeneity of the students. |
| Methodologies |
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Methodologies |
Description |
| Seminar |
The students will prepare a scientific presentation about a subject related to the mechanisms of gene expression regulation and will present it to the class. The presentation will be followed by a debate. |
| Laboratory practice |
Experimental work in the laboratory about genetic engineering and gene expression analysis. |
| Guest lecture / keynote speech |
Magister lectures about the principal topics of the learning program
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| Problem solving |
Learning based on problem solving. The students will have to solve a problem with the aid of previous information about the subject. |
| Objective test |
The exam will include questions based in multiple option selection and also problems. This will allow to modulate the final qualification of each student |
| Personalized attention |
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Methodologies
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| Seminar |
| Laboratory practice |
| Problem solving |
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| Description |
Students will be oriented before and during the preparation of seminars and the development of the practical course. They will involve interpretation of results. The problems and case solving will also need an orientation from the teachers.
Students with part-time dedication or waiver of presence should contact the teachers of the subject in the early going to establish a schedule of activities to acquire and evaluate in a complementary way the competences.
Titotial schedules:
Pfra. Esperanza Cerdán
Tuesday, wednesday and Thursday from 12.30 to 14.30
Pfra. Mª Angeles Freire
Monday 13-15 or previous appointment by e-mail. Some doubts may also be solved directly by e-mail.
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| Assessment |
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Methodologies
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Competencies |
Description
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Qualification
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| Seminar |
A5 A6 A9 A10 A11 B3 B5 B6 B9 C3 C8 |
Students give their peers a seminar about sppecific aspects of work of other scientists on an issue related to regulation of gene expression.
Half-presential students will have a specific activity combining the seminar contents and the problem solving
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15 |
| Laboratory practice |
A1 A2 A3 B1 B2 |
The collection and management of information from databases and scientific suits available on the web. A practical case will be carried out by students.
It will also be a laboratory session focussed on a transcriptional regulation experiment.
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25 |
| Guest lecture / keynote speech |
A5 A6 A9 A10 A11 |
Althought the theorethical knowledges will be teached and ellaborated in magister clases, we will take into consideration the assistence to the theorethical clases and the student´s participation.
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10 |
| Problem solving |
A13 B1 B2 |
We will present problems on different subjects related with the gene expression regulation to verify if the students are able to use the infomation that was given to them, or other that they find, in order to solve them.
Half-presential students will have a specific activity combining the seminar contents and the problem solving
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25 |
| Objective test |
A5 A6 A9 A10 A11 A13 |
It will consist in an exam that may include multiple answer questions, or case solving and will allow to mudulate the student final evaluation note.
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25 |
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Assessment comments |
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Half-presential students will have a specific activity combining the seminar contents and the problem solving (40%). Students with part-time dedication or waiver attendance may choose to be evaluated in a final exam if they do not qualify for continuous evaluation.
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| Sources of information |
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Basic
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Lodisch et al., (2005). Biología Molecular de la célula . Panamericana
Watson, Baker, Bell et al., (2006). Biología Molecular del Gen, 5º Ed. Panamericana
Lodish, Berk, et al (2013). Molecular and Cellular Biology 7th Ed. WH Freeman
Meister, G. (2011). RNA Biology. Wiley-VCH
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Articles and specialized texts will be updated in the moodle platform. |
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Complementary
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-Cheng B. and David H. Price Properties of RNA Polymerase II Elongation Complexes Before and After the P-TEFb-mediated Transition into Productive Elongation. JBC. 282, 21901–21912. 2007.
-Sims, R.J.; Belotserkovskaya R. and Reinberg, D. Elongation by RNA polymerase II: the short and long of it”. Genes & Dev.18, 2437-2468.2004.
-Wäle S. and Kehlenbach RH. The part and the whole: Functions of Nucleoporins in nucleocytoplasmic transport. Trends in Cell Biol 20: 461-469. 2010.
-Simpson, G.G., Dijwel, P.P., Quesada, V., Henderson, I. and Dean, C. “FY is an RNA 3´end-processing factor that interacts with FCA to control the Arabidopsis floral transition.” Cell 13, 777-797. 2003.
-Ghazy, M.A., He, X., Singh, B.N., Hampsey, M. and Moore C.>The essential N terminus of the Pta1 scaffold protein is required for snoRNA transcription termination and Ssu72 function but is dispensable for pre-mRNA 3´-end processing.” Mol. Cell Biol 29, 2296-2307. 2009.
-Graber, J.H., McAllister, G.D. and Smith, T.F.“Probabilistic prediction of Saccharomyces cerevisiae mRNA 3´-processing sites.” Nucleic Acids Res. 1851-1858. 2002.
-Bently, D. “Rules of engagement: co-transcriptional recruitment of pre-mRNA processing factors.” Curr. Opin. Cell Biol. 17, 251-256. 2005.
-Murchison, E. P. and Hannon, G.J. “miRNAs on the move: miRNA biogenesis and the RNAi machinery”Current Opinion in Cell Biology 16, 223–229.2004.
-Wang, Y., Chih Long Liu, John D. Storey, Robert J. Tibshirani, Daniel Herschlag, and Patrick O. Brown. “Precision and functional specificity in mRNA decay”. PNAS 99, 5860–5865. 2002.
-James E.C. Jepson Robert A. Reenan “RNA editing in regulating gene expression in the brain.” Biochimica et Biophysica Acta 1779, 459–470.2008.
Wu, H., Neilson, J.R., Kumar,Manocha, M.,Shankar, P.,Sharp, P.A. and Manjunath, miRNA Profiling of Naý¨ve, Effector and Memory CD8 T Cells>.” PloS One 10 | e1020. |
| Recommendations |
| Subjects that it is recommended to have taken before |
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| Subjects that are recommended to be taken simultaneously |
| Molecular Techniques/610441002 | | Advanced Cellular Biology/610441003 | | Molecular Microbiology /610441010 | | Protein Structure and Dynamics/610441011 | | Bioinformatics and Biomolecular models /610441020 |
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| Subjects that continue the syllabus |
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| Other comments |
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Is important that the students attend to the personal titorials to solve doubts. |
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