Identifying Data 2019/20
Subject (*) Molecular Microbiology Code 610441010
Study programme
Mestrado Universitario en Bioloxía Molecular , Celular e Xenética
Descriptors Cycle Period Year Type Credits
Official Master's Degree 2nd four-month period
First Optional 3
Language
Spanish
Teaching method Face-to-face
Prerequisites
Department Bioloxía
Departamento profesorado máster
Coordinador
Cid Blanco, Angeles
E-mail
angeles.cid@udc.es
Lecturers
Bou Arevalo, Germán
Cid Blanco, Angeles
Poza Domínguez, Margarita
Tomas Carmona, Maria Del Mar
E-mail
angeles.cid@udc.es
margarita.poza.dominguez@correo.udc.es
maria.tomas@udc.es
Web
General description PENDIENTE DE INCLUIR POR LOS SERVICIOS DE GADU LOS SIGUIENTES PROFESORES DEL INIBIC:
Germán Bou Arévalo (germanbou@canalejo.org)
Margarita Poza Domínguez (Margarita.Poza.Dominguez@sergas.es)
Mª del Mar Tomas Carmona (ma.del.mar.tomas.carmona@sergas.es)
Contingency plan

Study programme competencies
Code Study programme competences
A1 Skills of using usual techniques and instruments in the cellular, biological and molecular research: that are able to use techniques and instruments as well as understanding potentials of their uses and applications.
A2 Skills of working in a sure way in the laboratories knowing operation handbooks and actions to avoid incidents of risk.
A5 Skills of understanding the microorganisms' role as pathogenic agents and as biotechnological tools.
B1 Analysis skills to understand biological problems in connection with the Molecular and Cellular Biology and Genetics.
B2 Skills of decision making for the problem solving: that are able to apply theoretical knowledges and practical acquired in the formulation of biological problems and the looking for solutions.
B3 Skills of management of the information: that are able to gather and to understand relevant information and results, obtaining conclusions and to prepare reasoned reports on scientific and biotechnological questions
B4 Organization and work planning skills: that are able to manage the use of the time as well as available resources and to organize the work in the laboratory.
B5 Correct oral and written communication on scientific topics in the native language and at least in another International diffusion language.
B7 Personal progress skills : that are able to learn from freelance way, adapting to new situations, developing necessary qualities as the creativity, skills of leadership, motivation for the excellence and the quality.
B8 Critical reasoning skills and ethical commitment with the society: sensitivity in front of bioethical problems and to the ones related to the natural resource conservation
B9 Skills of preparation, show and defense of a work.
C3 Using ICT in working contexts and lifelong learning.
C4 Acting as a respectful citizen according to democratic cultures and human rights and with a gender perspective.
C5 Understanding the importanceof entrepreneurial culture and the useful means for enterprising people.
C6 Acquiring skills for healthy lifestyles, and healthy habits and routines.
C7 Developing the ability to work in interdisciplinary or transdisciplinary teams in order to offer proposals that can contribute to a sustainable environmental, economic, political and social development.
C8 Valuing the importance of research, innovation and technological development for the socioeconomic and cultural progress of society.

Learning aims
Learning outcomes Study programme competences
Understand the microbial cooperative behavior and the interactions of micro-organisms with other living beings at the molecular level AR5
BR5
BR7
BR8
CC6
CC7
CC8
Handle the techniques and understand the molecular basis of the fight against infections and resistance mechanisms AR1
AR2
AR5
BR1
CC4
CC7
CC8
Apply the molecular knowledge to understanding and solving problems BR1
BR2
BR3
BR4
BR7
BR8
BR9
CC3
CC4
CC5
CC6
CC7
CC8

Contents
Topic Sub-topic
Microbial cooperative behaviour
-Molecular basis for the cooperation
-Practical implications
Microbial interactions -Positive and negative interactions
-Molecular basis of the interactions with other microorganisms, plants or animals
Mechanisms of resistance to antimicrobial agents -Enzymes degrading antimicrobial agents
-Expulsion pumps
-Modification of targets
-Regulation of porins
New anti-infectious therapies
-Phagotherapy against multi-resistant bacteria
-Antitolerants
Bacterial tolerance and persistence -Phenotypic studies
-Molecular mechanisms
Practical study of different aspects involved in the resistance to antimicrobial agents -PCR of involved genes
-Gene clonning
-Protein expression
-Preparation of knock-out mutants
-Studies of the regulation of the mechanisms of resistance through RNA analysis

Planning
Methodologies / tests Competencies Ordinary class hours Student’s personal work hours Total hours
Short answer questions B1 B2 B5 2 0 2
Guest lecture / keynote speech A5 C4 C5 C8 14 35 49
Laboratory practice A2 B4 C6 C7 7 7 14
Seminar A1 B3 B7 B8 B9 C3 1 7 8
 
Personalized attention 2 0 2
 
(*)The information in the planning table is for guidance only and does not take into account the heterogeneity of the students.

Methodologies
Methodologies Description
Short answer questions Written test that will assess the grade of knowledge and understanding achieved by the student.
Guest lecture / keynote speech Exposure by the teaching staff of the theoretical basis of the subject
Laboratory practice Case study in the research laboratory of dfferent aspects involved in resistance to antimicrobial agents carried out by the students.
Seminar Working Group that will discuss certain aspects related to the subject, elaborating final conclusions

Personalized attention
Methodologies
Guest lecture / keynote speech
Laboratory practice
Seminar
Description
During the development of the subject will be addressed in the needs of the student and consultations relating to the subject matter, providing you the necessary support, both in person or through email.


Assessment
Methodologies Competencies Description Qualification
Guest lecture / keynote speech A5 C4 C5 C8 Avalíase pola proba de resposta breve 0
Laboratory practice A2 B4 C6 C7 Continuous assessment of practices 25
Seminar A1 B3 B7 B8 B9 C3 Active participation in the programmed seminars 5
Short answer questions B1 B2 B5 Written test on the knowledge acquired during the course, both in its theoretical and practical aspects 70
 
Assessment comments
Attendance is mandatory laboratory practices to be evaluated.
To account for the final grade in the value obtained in sections of seminars, practical and oral presentation, the student must have passed the short answer questions, corresponding to the theory of the subject.
The students that not pass the course at the first choice, must overcome the unapproved part at the second chance.
In the case of very exceptional circumstances and properly justified, the Professor could exempt total or partially to the student in that concur of any process of evaluation. This Student would have to subjected it a particular examination that will not leave doubts envelope his level of knowledge, competitions, skills and habilities.
"NO PRESENTADO" mark is obtained only when the student has not been submitted to the mixed test.
If the number of "Matrículas de Honor" (Distinction Award) that can be granted in the first option, you will not be granted in the second chance even when the maximum score is reached.


Sources of information
Basic Gerischer (Ed) (2008). Acinetobacter Molecular Biology. Caister Academic Press
Madigan, Martinko, Bender, Buckley y Stahl (2015). Brock. Biología de los microorganismos. 14ª edición. Pearson Educación, S.A.
Lederberg & Schaeter (Eds) (2009). Encyclopedia of Microbiology. 3rd edition. Academic Press

Complementary Otero, Muñoz, Bernárdez & Fábregas (2005). "Quorum sensing": El lenguaje de las bacterias. Zaragoza. Acribia
Maragakis & Perl (2008). Acinetobacter baumannii: epidemiology, antimicrobial resistance, and treatment options. Clin Infect Dis 46(8): 1254-63
Vila, Martí & Sánchez-Céspedes (2007). Porins, efflux pumps and multidrug resistance in Acinetobacter baumannii. J Antimicrob Chemother 59(6): 1210-5
Gootz (2010). The global problem of antibiotic resistance. Crit Rev Inmunol 30(1): 79-93
Pachón & Vila (2009). Treatment of multiresistant Acinetobacter baumannii infections. Curr Opin Invest Drugs 10(2): 150-6

It is noted here several revisions directly related to the content of the subject. In addition, during the development of the subject the student is given another bibliography, depending on the scheduled seminars and any other developments that arose.


Recommendations
Subjects that it is recommended to have taken before
Regulation of gene expression/610441006
Molecular Plant-Pathogen Interaction Mechanisms/610441018

Subjects that are recommended to be taken simultaneously

Subjects that continue the syllabus
Cellular Techniques/610441001
Molecular Techniques/610441002
Advanced Cellular Biology/610441003
Cell Signaling/610441004
Genetic Variation Mechanisms/610441005
Regulation of gene expression/610441006

Other comments
Of all the subjects that it recommends to have studied previously, compulsory all of them of the master's degree, the technical subjects are considered to be fundamental. The student has access to teacher presentations via Moodle, being these presentations only a guide for the study but never will be the total content of the matter.


(*)The teaching guide is the document in which the URV publishes the information about all its courses. It is a public document and cannot be modified. Only in exceptional cases can it be revised by the competent agent or duly revised so that it is in line with current legislation.