Identifying Data 2019/20
Subject (*) Recombinant proteins and protein Engineering Code 610441012
Study programme
Mestrado Universitario en Bioloxía Molecular , Celular e Xenética
Descriptors Cycle Period Year Type Credits
Official Master's Degree 2nd four-month period
 First Optional 3
Language
Spanish
Teaching method Face-to-face
Prerequisites
Department Bioloxía
Coordinador
Gonzalez Siso, Maria Isabel
 E-mail
isabel.gsiso@udc.es
Lecturers
Becerra Fernandez, Manuel
Gonzalez Siso, Maria Isabel
Vizoso Vázquez, Ángel José
 E-mail
manuel.becerra@udc.es
isabel.gsiso@udc.es
a.vizoso@udc.es
Web
General description A importancia actual dos procesos enzimáticos aplicados á industria alimentaria ou farmacolóxica e tal, que permite a producción de compostos que non poderían obterse de ningún outro xeito. A producción industrial de enzimas e un negocio que a comenzos do século XXI move en torno a 1600 millóns de dolares ao ano.
A utilización de enzimas en procesos industriais vese limitada en ocasións por factores inherentes á natureza das enzimas como por exemplo a súa falla de estabilidade fronte a condicións extremas de temperatura ou pH, a súa desnaturalización en presencia de solventes orgánicos ou a súa escasa actividade fronte a determinados sustratos. Na actualidade hay un amplo abano de técnicas de expresión e de Enxenería de Proteínas que permiten a xeración de proteínas modificadas co obxectivo de subsanar estas limitacións. Existe unha ampla gama de productos desenvolvidos por estas vías que se empregan en diversos campos.
Nesta asignatura describiranse métodos actuais para a expresión e modificación de proteínas, de uso tanto en investigación básica como en aplicacións biotecnolóxicas.

Study programme competencies
Code Study programme competences
A10 Skills of modifying genes, proteins and chromosomes with biotechnological applications
B3 Skills of management of the information: that are able to gather and to understand relevant information and results, obtaining conclusions and to prepare reasoned reports on scientific and biotechnological questions
B7 Personal progress skills : that are able to learn from freelance way, adapting to new situations, developing necessary qualities as the creativity, skills of leadership, motivation for the excellence and the quality.
C3 Using ICT in working contexts and lifelong learning.
C8 Valuing the importance of research, innovation and technological development for the socioeconomic and cultural progress of society.

Learning aims
Learning outcomes Study programme competences
Ability to learn and use biochemical concepts, techniques and resources available in databases related to the subject AR10
 BR7
 CC3
CC8
Ability to solve practical cases through the acquisition of skills that allow to carry out a simulated project of expression of recombinant proteins and directed evolution of proteins. AR10
 BR3
BR7
 CC3
CC8

Contents
Topic Sub-topic
Systems for expresión of native and recombinant proteins: bacterias Systems of expression of Heterologous proteins in bacteria and purification.
Systems for expresión of native and recombinant proteins: yeasts Systems of expression of Heterologous proteins in yeast and down-stream processing.
Systems for expresión of native and recombinant proteins: animal cells Genetic manipulation of animal cells. Systems of expression and production of proteins in mammalian cells.
Protein engineering I Introduction. Site-directed mutagenesis techniques.
Protein engineering II Techniques of artificial evolution of proteins.
Protein engineering III Techniques of stabilization and immobilization of enzymes.
Industrial applications of protein engineering Applications in Enzymology, pharmaceutical, food industry and other applications.

Planning
Methodologies / tests Competencies Ordinary class hours Student’s personal work hours Total hours
Guest lecture / keynote speech A10 B7 7 7 14
Laboratory practice A10 B3 C3 7 14 21
Mixed objective/subjective test B3 2 16 18
Directed discussion B3 C3 C8 7 14 21
 
Personalized attention 1 0 1
 
(*)The information in the planning table is for guidance only and does not take into account the heterogeneity of the students.

Methodologies
Methodologies Description
Guest lecture / keynote speech Oral presentation complemented with the use of audiovisual media in order to pass on knowledge and facilitate learning.
Laboratory practice A methodology that allows students to learn effectively through practical activities.
Mixed objective/subjective test Exam comprising questions type of testing trial, questions objective type testing and resolution of cases and problems.
Directed discussion Technique of group dynamics in which the members of a group discussed free, informal and spontaneous way on a subject, coordinated by a moderator.

Personalized attention
Methodologies
Directed discussion
Description
The directed discussion is conceived as moments of face-to-face student work with the teacher by involving compulsory student participation.

Assessment
Methodologies Competencies Description Qualification
Mixed objective/subjective test B3 Test to evaluate the knowledge acquired during the master classes, practical classes of laboratory as well as directed discussion
 60
Laboratory practice A10 B3 C3 Regular attendance and active participation to laboratory practices as well as the report made by students will be evaluated


 20
Directed discussion B3 C3 C8 Regular attendance and active participation will be evaluated

 20
 
Assessment comments

To get honours preference will be given to the best notes of the call of
June


Sources of information
Basic

-Cerdán Villanueva, M. E. Curso Avanzado de Proteínas y Ácidos Nucleicos. A Coruña. Universidade da Coruña. 2005. Libro.

-Cerdán Villanueva, M. E., Freire Picos, M. A., González Siso, M. I. y Rodríguez Torres, A. M., Biología Molecular. Avances y Técnicas generales , A Coruña. Universidade da Coruña, 1997, Libro.

-Gerd Gellisen Ed., Production of recombinant proteins: novel microbial and eukaryotic expression systems, Weinheim: Wiley-VCH, 2005, Libro,BM-720

-Glick, B. R., Molecular Biotechnology: Principles and Application of Recombinant DNA, Washington: American Society Microbiology, 2003, Libro,BM-668

-Gómez-Moreno, C. y Sancho, J. Estructura de proteínas. Ariel Ciencia. 2003. Libro

-González Siso, M. I., La Biotecnología en el tratamiento de residuos industriales , A Coruña. Universidade da Coruña. Servicio de Publicacións, 1999, Libro,

- Lutz, S., Bornscheuer. Protein Engineering Handbook. Wiley-Vch. Volumen 1 y 2. 2009. Libro. BM-785

-Ninfa, A. J., Fundamental laboratory approaches for biochemistry and biotechnology, Hoboken: John Wiley and Sons, 2010, Libro,BM-801

-Perera, J., Tormo, A., García, J. L., Ingeniería Genética. Vol I. Preparación, análisis, manipulación y clonaje del DNA. , Madrid. Síntesis , 2002, Libro,

-Perera, J., Tormo, A., García, J. L., Ingeniería Genética. Vol II. Expresión de DNA en sistemas heterólogos., Madrid. Síntesis , 2002, Libro,

-Thiel, T., Bissen, S. T., Lyons, E. M., Biotechnology: DNA to Protein. A Laboratory Project in Molecular Biology. , , 2001, Libro,

-Wink, M., An introduction to molecular Biotechnology: from molecular biological fundamentals to methods and applications in modern biotechnology, Verlag Chemie, GmbH, 2006, Libro,BM-762

Complementary


Recommendations
Subjects that it is recommended to have taken before
Molecular Techniques/610441002

Subjects that are recommended to be taken simultaneously
Protein Structure and Dynamics/610441011
Bioinformatics and Biomolecular models /610441020

Subjects that continue the syllabus
Project/610441022

Other comments


(*)The teaching guide is the document in which the URV publishes the information about all its courses. It is a public document and cannot be modified. Only in exceptional cases can it be revised by the competent agent or duly revised so that it is in line with current legislation.