Identifying Data 2019/20
Subject (*) Proteomics Code 610441013
Study programme
Mestrado Universitario en Bioloxía Molecular , Celular e Xenética
Descriptors Cycle Period Year Type Credits
Official Master's Degree 2nd four-month period
First Optional 3
Language
Spanish
Galician
English
Teaching method Face-to-face
Prerequisites
Department Bioloxía
Departamento profesorado máster
Coordinador
Cerdan Villanueva, Maria Esperanza
E-mail
esper.cerdan@udc.es
Lecturers
,
Calamia , Valentina
Cerdan Villanueva, Maria Esperanza
Fernández Puente, Patricia
Ruiz Romero, Cristina
E-mail
l.lourido@udc.es
esper.cerdan@udc.es
patricia.fernandez.puente@udc.es
cristina.ruiz.romero@correo.udc.es
Web
General description Coordina María Esperanza Cerdán Villanueva (esper.cerdan@udc.es)

PENDIENTE DE INCLUIR POR LOS SERVICIOS DE GADU LOS SIGUIENTES PROFESORES DEL INIBIC:
Dr. Fco. Javier Blanco García (Francisco_Blanco@canalejo.org)
Dra. Cristina Ruis Romero (crisruiz@canalejo.org)
Dra. Valentina Calamia


En esta materia se pretende formar al alumno para
.-Comprender las técnicas básicas de trabajo en proteómica
.-Obtener y manejar muestras de proteínas
.-Conocer las técnicas para la separación y detección masiva de las proteínas
.-Comprender métodos de análisis de datos proteómicos a gran escala
.-Conocer las aplicaciones de la proteómica en investigación básica, aplicada y clínica
.-La lectura y comprensión crítica de publicaciones científicas del campo de la
proteómica
Contingency plan

Study programme competencies
Code Study programme competences
A1 Skills of using usual techniques and instruments in the cellular, biological and molecular research: that are able to use techniques and instruments as well as understanding potentials of their uses and applications.
A3 Skills of understanding the functioning of cells through the structural organization, biochemistry, gene expression and genetic variability.
A9 Skills of understanding the structure and dynamics of proteins to individual and proteomic level, as well as the techniques that are necessary to analyze them and to study their interactions with other biomolecules.
B1 Analysis skills to understand biological problems in connection with the Molecular and Cellular Biology and Genetics.
B3 Skills of management of the information: that are able to gather and to understand relevant information and results, obtaining conclusions and to prepare reasoned reports on scientific and biotechnological questions

Learning aims
Learning outcomes Study programme competences
In this course knowledge and skills about the extraction, purification and characterization of proteins from biological systems is adquired. AR1
AR3
AR9
BR1
BR3

Contents
Topic Sub-topic
Proteomics 1.-The concept of proteomics and its applications. 2.-Preparation of protein extracts and protein solubilization.
3.-Proteomics by two-dimensional electrophoresis. 4.-Handling two-dimensional proteomics bioinformatics programs.
5.-Identification and characterization of proteins in micro-scale.
Differential expression proteomics: DIGE.
6.-Protein expression and protein chips.
7.-Protein identification by peptide mass fingerprinting.
8.-Tandem mass spectrometry (MS/MS):
peptide sequencing.
9.- Databases and search programs for
assisted protein identification by MS.
10.-Proteomics differential expression without
gel: ICAT, iTRAQ, SILAC.
11.-Applications of proteomics in the field of
Biomedicine.
12.-The human proteome.

Planning
Methodologies / tests Competencies Ordinary class hours Student’s personal work hours Total hours
Guest lecture / keynote speech A9 9 18 27
Laboratory practice A1 A3 A9 B1 B3 9 0 9
Objective test A1 A3 A9 B1 B3 2 37 39
 
Personalized attention 0 0
 
(*)The information in the planning table is for guidance only and does not take into account the heterogeneity of the students.

Methodologies
Methodologies Description
Guest lecture / keynote speech Magistral exposures
Laboratory practice A guided tour of techniques at the Proteomic unit
Objective test Questionary about the program content

Personalized attention
Methodologies
Guest lecture / keynote speech
Laboratory practice
Description
Students with part-time dedication or waiver of presence should contact the teachers of the subject in the early going to establish a schedule of activities to acquire and evaluate in a complementary way the competences.

Assessment
Methodologies Competencies Description Qualification
Guest lecture / keynote speech A9 Attendance and participation
15
Laboratory practice A1 A3 A9 B1 B3 Attendance and participation
15
Objective test A1 A3 A9 B1 B3 Multiple options selection/test
50
 
Assessment comments

Students with part-time dedication or waiver attendance may choose to
be evaluated in a final exam if they do not qualify for continuous evaluation.


Sources of information
Basic

To be specified in the on-line application(Moodle)

Complementary

Se especificarán en la aplicación de la materia


Recommendations
Subjects that it is recommended to have taken before

Subjects that are recommended to be taken simultaneously

Subjects that continue the syllabus

Other comments


(*)The teaching guide is the document in which the URV publishes the information about all its courses. It is a public document and cannot be modified. Only in exceptional cases can it be revised by the competent agent or duly revised so that it is in line with current legislation.