Identifying Data 2022/23
Subject (*) Proteomics Code 610441014
Study programme
Máster Universitario en Bioloxía Molecular, Celular e Xenética
Descriptors Cycle Period Year Type Credits
Official Master's Degree 2nd four-month period
 First Optional 3
Language
Spanish
Galician
English
Teaching method Face-to-face
Prerequisites
Department Bioloxía
Departamento profesorado máster
Coordinador
Cerdan Villanueva, Maria Esperanza
 E-mail
esper.cerdan@udc.es
Lecturers
Calamia , Valentina
Cerdan Villanueva, Maria Esperanza
Fernández Puente, Patricia
Lourido Salas, Lucía María
Ruiz Romero, Cristina
 E-mail
valentina.calamia@sergas.es
esper.cerdan@udc.es
patricia.fernandez.puente@udc.es
l.lourido@udc.es
cristina.ruiz.romero@correo.udc.es
Web
General description Está coordinado por María Esperanza Cerdán Villanueva (esper.cerdan@udc.es) e é impartido por profesores INIBIC (contacto: cristina.ruiz.romero@sergas.es)
O obxectivo desta materia é formar ao alumno para:
.-Comprender as técnicas básicas de traballo en proteómica
.-Obter e xestionar mostras de proteínas
.-Coñecer as técnicas para a separación e detección masiva de proteínas
.-Comprender métodos de análise de datos proteómicos a gran escala
.-Coñecer as aplicacións da proteómica na investigación básica, aplicada e clínica
.-A lectura e comprensión críticas de publicacións científicas no campo da
proteómica

Study programme competencies
Code Study programme competences
A2 Skills of using usual techniques and instruments in the cellular, biological and molecular research: that are able to use techniques and instruments as well as understanding potentials of their uses and applications.
A3 Skills of understanding the functioning of cells through the structural organization, biochemistry, gene expression and genetic variability.
A9 Skills of understanding the structure and dynamics of proteins to individual and proteomic level, as well as the techniques that are necessary to analyze them and to study their interactions with other biomolecules.
B1 Analysis skills to understand biological problems in connection with the Molecular and Cellular Biology and Genetics.
B3 Skills of management of the information: that are able to gather and to understand relevant information and results, obtaining conclusions and to prepare reasoned reports on scientific and biotechnological questions
C1 Ability to express oneself correctly, both orally and in writing, in the official languages of the autonomous community
C2 Ability to know and use appropriately the technical terminology of the field of knowledge of the master, in the native language and in English, as a language of international diffusion in this field

Learning aims
Learning outcomes Study programme competences
In this course knowledge and skills about the extraction, purification and characterization of proteins from biological systems is adquired. AR2
AR3
AR9
 BR1
BR3
 CC1
CC2

Contents
Topic Sub-topic
Proteomics 1.-The concept of proteomics and its applications. 2.-Preparation of protein extracts and protein solubilization.
3.-Proteomics by two-dimensional electrophoresis. 4.-Handling two-dimensional proteomics bioinformatics programs.
5.-Identification and characterization of proteins in micro-scale.
Differential expression proteomics: DIGE.
6.-Protein expression and protein chips.
7.-Protein identification by peptide mass fingerprinting.
8.-Tandem mass spectrometry (MS/MS):
peptide sequencing.
9.- Databases and search programs for
assisted protein identification by MS.
10.-Proteomics differential expression without
gel: ICAT, iTRAQ, SILAC.
11.-Applications of proteomics in the field of
Biomedicine.
12.-The human proteome.

Planning
Methodologies / tests Competencies Ordinary class hours Student’s personal work hours Total hours
Guest lecture / keynote speech A9 C2 9 18 27
Laboratory practice A2 A3 A9 B1 B3 C1 9 0 9
Objective test A2 A3 A9 B1 B3 2 37 39
 
Personalized attention 0 0
 
(*)The information in the planning table is for guidance only and does not take into account the heterogeneity of the students.

Methodologies
Methodologies Description
Guest lecture / keynote speech Magistral exposures
Laboratory practice A guided tour of techniques at the Proteomic unit
Objective test Questionary about the program content

Personalized attention
Methodologies
Guest lecture / keynote speech
Laboratory practice
Description
Students with part-time dedication or waiver of presence should contact the teachers of the subject in the early going to establish a schedule of activities to acquire and evaluate in a complementary way the competences.

Assessment
Methodologies Competencies Description Qualification
Guest lecture / keynote speech A9 C2 Attendance and participation
 25
Laboratory practice A2 A3 A9 B1 B3 C1 Attendance and participation
25
Objective test A2 A3 A9 B1 B3 Multiple options selection/test
 50
 
Assessment comments

Students with part-time dedication or waiver attendance may choose to
be evaluated in a final exam if they do not qualify for continuous evaluation.

Sources of information
Basic

To be specified in the on-line application(Moodle)
Complementary

Se especificarán en la aplicación de la materia

Recommendations
Subjects that it is recommended to have taken before

Subjects that are recommended to be taken simultaneously

Subjects that continue the syllabus

Other comments


(*)The teaching guide is the document in which the URV publishes the information about all its courses. It is a public document and cannot be modified. Only in exceptional cases can it be revised by the competent agent or duly revised so that it is in line with current legislation.