Identifying Data 2019/20
Subject (*) Molecular Plant-Pathogen Interaction Mechanisms Code 610441018
Study programme
Mestrado Universitario en Bioloxía Molecular , Celular e Xenética
Descriptors Cycle Period Year Type Credits
Official Master's Degree 2nd four-month period
First Optional 3
Language
Spanish
Galician
English
Teaching method Face-to-face
Prerequisites
Department Bioloxía
Coordinador
Diaz Varela, Jose
E-mail
jose.diaz.varela@udc.es
Lecturers
Diaz Varela, Jose
Silvar Pereiro, Cristina
E-mail
jose.diaz.varela@udc.es
c.silvar@udc.es
Web
General description Esta materia trata os aspectos moleculares da interacción da planta cos patóxenos e, en menor medida, das interaccións relacionadas con outros organismos (herbívoros, rizobios e micorrizas)

Study programme competencies
Code Study programme competences
A4 Skills to apply molecular techniques to the study of the plant cell physiology, its response to external triggers and their biotechnological applications.
A5 Skills of understanding the microorganisms' role as pathogenic agents and as biotechnological tools.
A6 Skills of understanding the functioning of cells through the structural organization, biochemistry, gene expression and genetic variability.
A8 Skills of having an integrated view of the previously acquired knowledge about Molecular and Cellular Biology and Genetics, with an interdisciplinary approach and experimental work.
B3 Skills of management of the information: that are able to gather and to understand relevant information and results, obtaining conclusions and to prepare reasoned reports on scientific and biotechnological questions
B5 Correct oral and written communication on scientific topics in the native language and at least in another International diffusion language.
B9 Skills of preparation, show and defense of a work.

Learning aims
Learning outcomes Study programme competences
- To understand the molecular mechanisms of plant-pathogen interaction AR4
AR5
- To know the different mechanisms of the plant response to pathogens. AR4
AR5
AR6
AR8
To understand and be able to use the experimental approaches to research in this field. AR4
AR5
BR3
BR5
- Ability for critically reviewing scientific papers related to this subject. AR5
AR6
BR3
BR5
BR9

Contents
Topic Sub-topic
Molecular mechanisms in plant-pathogen interaction. Recognition of the plant by the pathogen and mechanism to attack the plant.
Recognition of the pathogen by the plant amnd mechanisms of defense. Pathogen Associated Molecular Patterns (PAMPs). Oxidative burst. Salicylates, jasmonates and ethylene. Hypersensitive response. Gene-for-gene resistance. Nonhost resistance. Induced resistance to pathogens: SAR and ISR. Npr1. Priming. Transcription factors involved in resistance.
Other interactions related to plant-pathogen interaction. Recognition of herbivores, signalling and defense mechanisms. Rhizobium-plant interaction. Mycorrhizae.

Planning
Methodologies / tests Competencies Ordinary class hours Student’s personal work hours Total hours
Guest lecture / keynote speech A4 A5 A6 A8 12 30 42
Document analysis A5 A6 B3 B5 B9 2 10 12
Laboratory practice A4 A5 B3 B5 7 10.5 17.5
Objective test A4 A5 A6 A8 2.5 0 2.5
 
Personalized attention 1 0 1
 
(*)The information in the planning table is for guidance only and does not take into account the heterogeneity of the students.

Methodologies
Methodologies Description
Guest lecture / keynote speech Lectures about main contents of the subject, supported by presentations and videos. Presentation is combined with critical dialogue with the students about the topics.
Document analysis Reading and analysis of a primary research paper related to the subject, accompanied by its presentation in the classroom by the student and further discussion with the lecturer and the other students.
Laboratory practice Practicals related to the subject, consisting in experiments, followed by data analysis, discussion and writing of a report.
Objective test Exam about the topics of the lectures.

Personalized attention
Methodologies
Document analysis
Description
The students can attend, in the corresponding hours, to the lecturer´s office to ask any question about the subject, and particularly about the work to do.
For those students with official part-time dedication, the attendance to the lectures might be replaced by a written work, if the student requires it.

Assessment
Methodologies Competencies Description Qualification
Guest lecture / keynote speech A4 A5 A6 A8 Attendance and participation in the lectures. 10
Document analysis A5 A6 B3 B5 B9 Aspects to be assessed: Proper understanding of the paper by the student, the presentation in the classroom and the participation in the discussion in the classroom (including the critical review of the paper). 40
Laboratory practice A4 A5 B3 B5 Attendance and participation in the laboratory, as well as a written report. 20
Objective test A4 A5 A6 A8 Exam about the topics in the lectures. 30
 
Assessment comments

The students who pass the subject in the first opportunity, will be prefentially considered to get the highest qualification (with honors).

For those students who are semipresential or with official part-time dedication, the attendance to the lectures might be replaced by a written work, if the student requires
it.


Sources of information
Basic

Dickinson, M. 2003. Molecular Plant Pathology. Bios Scientific Publishers.

Hammond-Kosack, K.E. & Jones, J.D.G. 2015. Responses to plant pathogens. En: Buchanan, B.B., Gruissem, W. & Jones, R.L (eds.) "Biochemistry and molecular biology of plants" Capítulo 22, pp. 984-1050. Wiley-Blackwell-ASPB.

Smith, A.M., Cupland, G., Dolan, L., Harberd, N., Jones, J., Marin, C., Sablowski, R. & Amey, A.. 2009. Plant Biology. Garland Science. Capítulo 8.

Taiz, L., Zeiger, E., Moller, I.M. & Murphy, A. 2014. plant Physiology and development, Sixth Edition. Sinauer Associates, Inc. Capítulo 23.

Walters, D. R. 2011. Plant defense. Wiley-Blackwell.

 

Complementary

- Agrios, G. N. 2005. Plant pathology, 5ª Ed. Academic Press.

- Albersheim, P. Darvill, A., Roberts, K., Sederoff, R. & Staehelin, A.. 2010. Plant Cell Walls: from Chemistry to Biology. Garland Science. Capítulo 8.

- Dyakov, Y., Dzhavakhiya, V. & Korpela, T. 2007. Comprehensive and molecular phytopathology. Elsevier.

- Huang, J.-S. 2001. Plant pathogenesis and resistance: biochemistry and physiology of plant-microbe interactions. Kuwer Academic Publishers.

- Nuez, F., Pérez de la Vega, M. & Carrillo, J.M. 2004. Resistencia genética a patógenos vegetales. Univ. Politécnica de Valencia – Univ. de León.

- Pallás, V., Escobar, C., Rodríguez Palenzuela, P. & Marcos, J.F. 2008. Herramientas biotecnológicas en fitopatologia. Ed. Mundi-Prensa.

- Parker, J. 2009. Molecular aspects of plant disease resistance. Blackwell Publishing Ltd.

- Slusarenko, A. J., Fraser, R. S. S. & van Loon, L. C. 2000. Mechanisms of resistance to plant diseases. Kluwer Academic Publishers.

- Walters, D., Newton, A. & Lyon, G. 2007. Induced resistance for plant defence. A sustainable approach to crop protection. Blackwell Publishing.


Recommendations
Subjects that it is recommended to have taken before

Subjects that are recommended to be taken simultaneously
Plant Biotechnology/610441019

Subjects that continue the syllabus
Cellular Techniques/610441001
Molecular Techniques/610441002
Cell Signaling/610441004

Other comments


(*)The teaching guide is the document in which the URV publishes the information about all its courses. It is a public document and cannot be modified. Only in exceptional cases can it be revised by the competent agent or duly revised so that it is in line with current legislation.