Identifying Data 2018/19
Subject (*) Molecular Biology Code 610509117
Study programme
Mestrado Universitario en Investigación Química e Química Industrial (Plan 2017)
Descriptors Cycle Period Year Type Credits
Official Master's Degree Yearly
First Optional 3
Language
Spanish
Teaching method Face-to-face
Prerequisites
Department Bioloxía
Coordinador
Lamas Maceiras, Mónica
E-mail
monica.lamas@udc.es
Lecturers
Cerdan Villanueva, Maria Esperanza
Lamas Maceiras, Mónica
E-mail
esper.cerdan@udc.es
monica.lamas@udc.es
Web http://www.usc.es/gl/centros/quimica/curso/master.html
General description Esta materia contén conceptos básicos e fundamentais dos métodos de investigación sobre procesos biolóxicos que son executadas en células vivas .

Study programme competencies
Code Study programme competences
A1 Define concepts, principles, theories and specialized facts of different areas of chemistry.
A3 Innovate in the methods of synthesis and chemical analysis related to the different areas of chemistry
A4 Apply materials and biomolecules in innovative fields of industry and chemical engineering.
B1 Possess knowledge and understanding to provide a basis or opportunity for originality in developing and / or applying ideas, often within a research context
B4 Students should be able to communicate their conclusions, and the knowledge and the reasons that support them to specialists and non-specialists in a clear and unambiguous manner
B5 Students must possess learning skills to allow them to continue studying in a way that will have to be largely self-directed or autonomous.
B7 Identify information from scientific literature by using appropriate channels and integrate such information to raise and contextualize a research topic
B10 Use of scientific terminology in English to explain the experimental results in the context of the chemical profession
B11 Apply correctly the new technologies to gather and organize the information to solve problems in the professional activity.
C1 CT1 - Elaborar, escribir e defender publicamente informes de carácter científico e técnico
C3 CT3 - Traballar con autonomía e eficiencia na práctica diaria da investigación ou da actividade profesional.
C4 CT4 - Apreciar o valor da calidade e mellora continua, actuando con rigor, responsabilidade e ética profesional.

Learning aims
Learning outcomes Study programme competences
Knowing the basics for the isolation , cloning , expression and purification of proteins. Learn the basic techniques used to visualize biological processes in cell AC1
AC3
AC4
BC1
BC4
BC5
BC7
BC10
BC11
CC1
CC3
CC4

Contents
Topic Sub-topic
ITEM 1. Handling and DNA sequencing.
1. Sense of the subject (Introduction )
This topic will address the description of the main tools and methods to manipulate genetic information and verify that the operations have been successful and have been conducted as they are designed .
2. Wording of the subject.
Restriction enzymes and cloning vectors hybridization for the detection of specific sequences, PCR, DNA sequencing.
3. Bibliography
- Molecular Biology of the Cell fifth edition ( 2008 ) . Alberts et al. Garland Science. Chapter 8.
- Molecular Biology fourth edition (2008 ) . Robert F. Weaver. McGraw -Hill International Edition. Chapters 4 and 5.
4. Activities to develop.
During the development of the subject practical cases that students must solve using the knowledge gained will arise. It could also be considered that students will read scientific articles related to the topic for exhibition / public presentation.
ITEM 2. Techniques for obtaining and analyzing proteins.
1. Sense of the subject (Introduction)
We look at how, using the techniques seen in item 1, we can produce and purify proteins in the lab for testing its activity, determine its structure, etc.
2. Wording of the subject.
Recombinant proteins , labeling of proteins, protein expression and purification , and protein sequencing analysis .
3. Bibliography
- Molecular Biology of the Cell fifth edition ( 2008 ) . Alberts et al. Garland Science. Chapter 8.
- Molecular Biology fourth edition (2008 ). Robert F. Weaver. McGraw -Hill International Edition. Chapters 4 and 5.
4. Activities to develop.
During the development of the subject practical cases that students must solve using the knowledge gained will arise. It could also be considered that students will read scientific articles related to the topic for exhibition / public presentation.
ITEM 3. Viewing biological processes.
1. Sense of the subject (Introduction)
In this issue the main methods of display structures and biological processes, both in vivo and in vitro will be explained.
2. Wording of the subject.
Chemical markers , GFP and fluorescent fusion proteins , optical microscopy (confocal, super-resolution, real-time ), electron microscopy.
3. Bibliography
- Molecular Biology of the Cell fifth edition (2008). Alberts et al. Garland Science. Chapter 9.
4. Activities to develop.
During the development of the subject practical cases that students must solve using the knowledge gained will arise. Also scientific work to read an comment by the students.

Planning
Methodologies / tests Competencies Ordinary class hours Student’s personal work hours Total hours
Problem solving A1 A4 A3 B1 B5 B11 C3 7 10 17
Seminar B4 B7 B10 C1 C4 2 8 10
Mixed objective/subjective test A1 A4 B1 B4 C3 C4 1 36 37
Guest lecture / keynote speech B5 C4 11 0 11
 
Personalized attention 0 0
 
(*)The information in the planning table is for guidance only and does not take into account the heterogeneity of the students.

Methodologies
Methodologies Description
Problem solving Theoretical / practical class in which the teacher propose and students solve applications of theory, problems and applied questions. It may also imclude the presentation by students of a topic related to the subject.
Small group interactive classes seminars , " S " in the timetables)
Seminar Tutorials in very small group ("T" in the timetables ) planned by the teacher and coordinated by the Tutoring Center. In general, each student will account for two hours per semester and subject. Monitoring activities as directed exercises, clarification of doubts about theory, problems, exercises, readings or other proposed tasks. Also the presentation, discussion or comments in small groups. Attendance at these classes is mandatory.
Mixed objective/subjective test Test to evaluate skills and knowledge
Guest lecture / keynote speech Lesson taught by the teacher who may have different formats (theory, problems and / or general examples, general guidelines on the matter ... ). The teacher may have the support of audiovisual and computer media but, in general, students do not need to handle them in class.

Personalized attention
Methodologies
Problem solving
Seminar
Description
Students with part-time dedication or waiver of presence should contact the teachers of the subject in the early going to establish a schedule of activities to acquire and evaluate in a complementary way the competences.

Assessment
Methodologies Competencies Description Qualification
Guest lecture / keynote speech B5 C4 Evaluation of attendance and participation 10
Problem solving A1 A4 A3 B1 B5 B11 C3 Continuous assessment 5
Seminar B4 B7 B10 C1 C4 Continuous assessment 15
Mixed objective/subjective test A1 A4 B1 B4 C3 C4 Final evaluation 70
 
Assessment comments
<p class="tw-data-text vk_txt tw-ta tw-text-small" data-placeholder="Traducción" id="tw-target-text" data-fulltext="" dir="ltr">Evaluation procedure.
The assessment of this will be done through continuous assessment and the completion of a final exam.<br />The access to the exam requires participation in at least 80% of classroom and teaching compulsory attendance <br />activities (seminars and tutorials.
Continuous assessment (N1) will weigh 40% in the total of the course and consist of two components: <br />interactive small group classes (seminars) and interactive classes in very small groups (tutorials). <br />Seminars and tutorials may include exercises and assignments made in person, exercises submitted to the teacher<br />or public presentations of topics previously selected.
The final exam (N2) will cover the entire content.
The student's score, will be obtained as a result of applying the following formula:
End = maximum Note (0.4 x 0.6 x N1 + N2, N2)
N1 is the corresponding to the continuous assessment (scale 0-10) and N2 numerical final exam (0-10 scale numerical grade.</p><p class="tw-data-text vk_txt tw-ta tw-text-small" data-placeholder="Traducción" data-fulltext="" dir="ltr">Students with part-time dedication or waiver attendance may choose to
be evaluated in a final exam if they do not qualify for continuous evaluation.</p>

Sources of information
Basic

Basic

Molecular Biology of the Cell (Alberts y otros, Garland Science, sexta edición, 2015)

As reference

Molecular Biology (Weaver, McGraw Hill Higher Education, quinta edición, 2015)

Complementary


Recommendations
Subjects that it is recommended to have taken before

Subjects that are recommended to be taken simultaneously

Subjects that continue the syllabus

Other comments

It is necessary to know the different kinds of biomolecules and&amp;nbsp; processes of transfer of genetic information in cells. It is very important to attend the lectures. It is essential to keep the study of matter " up to day".



(*)The teaching guide is the document in which the URV publishes the information about all its courses. It is a public document and cannot be modified. Only in exceptional cases can it be revised by the competent agent or duly revised so that it is in line with current legislation.