.- In order to add all the qualifications and pass this subject it will be mandatory to acquire the minimum scores in the three evaluable parts INDEPENDENTLY: Activities (Problem solving / Document analysis), Practical Classes in the Laboratory and Objective Probe (or Final Examination).
The oficial final exams in June and in July will be presentially, unless in case of pandemic situation in which will use the Moodle platform only.

The continous assesments, previous to the oficial opportunities, will also be on line. Two partial exams will be issued.- For Final Qualifications or ACTAS (on any of the 2 opportunities, JUNE or JULY): The marks of the Objective Probe, Laboratory Practices, and Seminars will be added only if all of them reach 45% of their value. If this percentage is not reached, the final grade that will appear in ACTAS will be 4.

.-In the Final Exam of the 2nd Opportunity_(July), the student will be able to recover only the
theoretical. It will not be an exam for the Practical Part in this 2º Opportunity.

.-The attendance to Practical Laboratory Classes is a mandatory condition to be evaluated. Failure
to attend classes without a properly justified reason means failing the subject. The students who had passed the Practical Part in the two previous academic years may apply for a request for his validation as overcome (PASS).

.- The students that do not show up in any of the two official examination dates will obtain a NOT
PRESENTED in the Final Grades (ACTAS).

.-According to the rule of qualifications and records in Degrees and Masters, the Quality Committee of the Faculty of Sciences agreed to the recommendation to concede the “Honors Qualification” to those students who obtained the highest marks in the 1st Opportunity.

.-For students with part-time dedication or with an exemption of class assistance, in June and July, there will be a specific exam for overall assessment.

.- Exceptionally, in the case of those students that, for duly justified reasons, are not able to
perform all continuous assessment tests or evaluable activities, the professors will take their deems for appropriate measures.
.-Students with recognition of part-time dedication and academic exemption for attendance both in the end-of-term opportunity and in the second opportunity will take into account, for the calculation of the overall grade, the grade obtained in the theoretical exam and the corresponding practical part (see above format of both exams), representing these 75% and 25% of the final grade, respectively.

Learning outcomes	Study programme competences
The approach of the Master Classes is to improve the knowledge and the ability of reflection on a discipline that also, once in the professional field, will demand a good praxis and adhere to ethical principles. The Laboratory Classes are more focused on the expertise on know how to do and how to be, related to the field of the Biochemistry and Molecular Biology.	A8 A12 A17 A27 A29 A30 A31	B1 B2 B3 B4 B5 B7 B10 B11 B13

Topic	Sub-topic
1.-Basal Transcription	RNA polymerases, core promoter and general transcription factors.Transcription mechanism: initiation, elongation and termination. Methodology to study: transcription start site selection, transcriptional termination and interactions nucleic acids-proteins.
2.-Regulated transcription and chromatin involvement in transcriptional regulation	Activators and repressors. DNA binding domains: DNA-proteins interactions. Chromatin remodeling complexes. Acetilation, deacetilation and other histones modifications in the regulation of gene expression. Techniques to study transcriptional regulation. Regulation examples of specific genes
3.-RNA processing and coordination of co-transcriptional events in eukaryotes	RNA cleavage and polyadenylation. RNA splicing. Processing of ribosomic and transferent RNA
4.-RNA as regulator of gene expression	RNA edition. Control of mRNA quality. Function of snRNA and transcriptional regulation. sncRNAs and the gene silencing mechanism. Antisense RNA in the translational regulation and applications of RNA. RNomics aspects
5.-Protein Translation	General aspects. Ribosomes. Translation mechanism: Initiation, elongation and termination. Differences in eukaryotes. Translation in mitochondria. Translational inhibitors.
6.-Protein Processing	Postranslational modifications of proteins. Folding: Chaperones and Prions. Ubiquitination and SUMOilation. Programed degradation: Proteasome
7.-Protein Transport	Cotranslational and postranslational translocation. Classification and distribution of new synthesized proteins. Traffic nucleo-cytoplasm. Transport regulation and final destiny of proteins in the cell.
8.-Basics of Cell Signaling	Classification of intercellular communication. Stages of intracellular signaling. Organization of signaling and pathways. Signaling molecules: types and functions.
9.- Reception of external signals and intracellular transduction	Membrane and intracellular receptors: types and mechanisms of activation. Intercellular Messenger substances or Second Messengers, protein kinase cascades and signal transduction to nucleus.
10.- Examples of control mechanisms and coordination of cell physiological activities	Cell Growth and Proliferation: regulation of cell cycle, apoptosis and cancer. Cell Senescence Signaling.

Methodologies / tests	Competencies	Ordinary class hours	Student’s personal work hours	Total hours
Laboratory practice	A8 A12 A17 A27 A30 A31 B1 B2 B3 B4 B5 B7 B10 B13	15	22.5	37.5
Problem solving	A29 B1 B2 B3 B4 B5 B7 B10 B11 B13	7	17.5	24.5
Guest lecture / keynote speech	A29 B2 B3 B4 B7 B10 B11 B13	28	0	28
Document analysis	A29 B1 B3 B5 B7 B10 B11 B13	1	3	4
Mixed objective/subjective test	A29 B2 B3 B7 B10 B13	2.5	50	52.5

Personalized attention		3.5	0	3.5

(*)The information in the planning table is for guidance only and does not take into account the heterogeneity of the students.

Methodologies	Description
Laboratory practice	Focused on the study of gene expression, with the use of databases, with the analysis of reporter genes expression and/or with the study of protein expression.
Problem solving	This section will include the approach and resolution of problems of different aspects in small groups of students, combining the methodologies of problem-based learning and collaborative work.
Guest lecture / keynote speech	Oral Presentation complemented with audiovisual media to transmit knowledges and provide the learning. Besides it will improve the participation of the students.
Document analysis	Read and comprenhesion of research papers. Searches of information, analysis, discussion, participation of all group components documents elaboration and conclussions. Defense in seminars.
Mixed objective/subjective test	It will be used for the evaluation of the knowledge, skills, attitudes, and so, acquired by the student along the course, and will include different types of questions: multiple answer, short, etc.

Methodologies	Competencies	Description	Qualification
Laboratory practice	A8 A12 A17 A27 A30 A31 B1 B2 B3 B4 B5 B7 B10 B13	LABORATORY CLASSES: The assistance is mandatory. The students will interpret the obtained results. Besides they will present a work that will include a small research project based on the results in the practical course and an exam.	25
Mixed objective/subjective test	A29 B2 B3 B7 B10 B13	FINAL EXAMINATION: The knowledges obtained by the students in the Master and Small Group Classes will be evaluated in a final exam.	40
Problem solving	A29 B1 B2 B3 B4 B5 B7 B10 B11 B13	Resolution of problems, student's work in Reduced Groups: exercises, seminars (as indicated in document analyses) and possibility of small exams.	20
Document analysis	A29 B1 B3 B5 B7 B10 B11 B13	Small group activity: Activity of searching and handling of scientific information that will be used to elaborate activities of science dissemination, with a class exposition in small groups, and with an ending debate. The scientific rigor of information (group work), the quality of visual presentation (group work), the fluidity and clarity of oral exposure (individual work) and responsiveness (individual work) will be evaluated.	15

Assessment comments
.- In order to add all the qualifications and pass this subject it will be mandatory to acquire the minimum scores in the three evaluable parts INDEPENDENTLY: Activities (Problem solving / Document analysis), Practical Classes in the Laboratory and Objective Probe (or Final Examination). The oficial final exams in June and in July will be presentially, unless in case of pandemic situation in which will use the Moodle platform only. The continous assesments, previous to the oficial opportunities, will also be on line. Two partial exams will be issued.- For Final Qualifications or ACTAS (on any of the 2 opportunities, JUNE or JULY): The marks of the Objective Probe, Laboratory Practices, and Seminars will be added only if all of them reach 45% of their value. If this percentage is not reached, the final grade that will appear in ACTAS will be 4. .-In the Final Exam of the 2nd Opportunity_(July), the student will be able to recover only the theoretical. It will not be an exam for the Practical Part in this 2º Opportunity. .-The attendance to Practical Laboratory Classes is a mandatory condition to be evaluated. Failure to attend classes without a properly justified reason means failing the subject. The students who had passed the Practical Part in the two previous academic years may apply for a request for his validation as overcome (PASS). .- The students that do not show up in any of the two official examination dates will obtain a NOT PRESENTED in the Final Grades (ACTAS). .-According to the rule of qualifications and records in Degrees and Masters, the Quality Committee of the Faculty of Sciences agreed to the recommendation to concede the “Honors Qualification” to those students who obtained the highest marks in the 1st Opportunity. .-For students with part-time dedication or with an exemption of class assistance, in June and July, there will be a specific exam for overall assessment. .- Exceptionally, in the case of those students that, for duly justified reasons, are not able to perform all continuous assessment tests or evaluable activities, the professors will take their deems for appropriate measures. .-Students with recognition of part-time dedication and academic exemption for attendance both in the end-of-term opportunity and in the second opportunity will take into account, for the calculation of the overall grade, the grade obtained in the theoretical exam and the corresponding practical part (see above format of both exams), representing these 75% and 25% of the final grade, respectively.

Basic	Lodish, Berk, Matsudaria, Kaiser et al., (2008). Biología Celular y Molecular. Ed. Médica Panamericana Karp G. (2011). Biología Celular y Molecular. Conceptos y experimentos. McGraw-Hill Interamericana Eds., S.A. de C.V., traducción de la 6ª ed. de Cell and Molecular Biology Elliot, W.H. & Elliot, D.C. (2002). Bioquimica y Biologia Molecular. Ariel, S.A. Stryer,L, Berg, J.M. %Tymoczko, J.L. (2013). Bioquímica: con aplicaciones clínicas. Ed. Reverté, 7ª Ed. Lewin B. (2011). Genes X. Jones and Bartlett Publishers, LLC Bruce, Alberts [et al.]. (2008). Molecular biology of the cell. New York : Garland Science, 5th ed. Lodish, Berk, Krieger, Kaiser et al., (2013). Molecular Cell Biology. WhFreeman Whitford, D. (2005). Proteins: Structure and Function. John Wiley & Sons, Ltd. Meister G. (2011). RNA Biology. Wiley-VHH Herráez, A. (2012). Texto inlustrado de Biología Molecular e ingeniería genética. Elsevier
	In the Moodle we Will update links to web pages and other bibliographic sources. Contingency plan: no modifications since they are included in the Moodle.
Complementary	Krauss, Gerhard. (2008). Biochemistry of signal transduction and regulation.. Weinheim : Wiley-VCH. 2nd ed. Rhoads R. (2010). miRNA Regulation of the translational machinery. Springer Dalbey, R.E. & von Heijne, G. (2002). Protein targeting, transport & translocation. Academic Press Meyers, R.A. (2007). Proteins: from analytical to structural genomics (Volume I and II). Wiley-VCH Verlag GmbH & Co.