To pass the course, in any of the diets to which the student may go, the student must have obtained a 5 out of 10 in the different parts of the "mixed test", performing all tasks that are considered mandatory, and obtain a minimum score of 2 points on a maximum 4. To account for the final grade in the value obtained in sections of
seminars, practical and case study, the student must have passed
the mixed test, corresponding to the theory of the subject.

In order to be evaluated, students must attend to practical sessions. In the case of not passing the subject in a first option, in the second option, the student must pass only the part that was not passed.

For a student to be considered "NOT PRESENT", he must have the following requirements: not site the examination (the mixed-test) and not attend half of the practice sessions.

If the number of "with Honours" that may be granted is exhausted in the first option, none will be granted in the second option, even though the maximum note is obtained.

Learning outcomes	Study programme competences
Fluid handling of the basic techniques of microbiology laboratory and their potential applications in industry and research.	A1 A2 A9 A11 A13 A14 A15 A21 A25 A26 A27 A29 A30 A31	B2 B3 B4 B5 B6 B7 B8 B10 B11	C3 C6 C8
Ability to relate concepts and practical application thereof.		B1 B6 B8 B10 B12	C1

Topic	Sub-topic
I. Methods for detection and quantification of microorganisms	1. Sampling 2. Processing of samples 3. Methods of enrichment, isolation and culture 4. Methods of counting
II. Classification and identification of prokaryotes	1. Phenotypic methods 2. Genotypic methods
III. Measures of biomass and microbial metabolic activity	1. Estimates of the total microbial biomass 2. Specific determination of biomass 3. Measures of microbial activity
PRACTICES	1. Methods of counting and estimating biomass and microbial activity 2. Microbiological analysis of different materials 3. Determination of indicator and pathogen microorganisms 4. Rapid bacterial identification phenotypic techniques 5. Genotypic methods for analysis of microorganisms
SEMINARS	1. Reporting of results 2. Conducting case studies

Methodologies / tests	Competencies	Ordinary class hours	Student’s personal work hours	Total hours
Guest lecture / keynote speech	A1 A14 A15 A21 A25 A29 B12 C6 C8	8	24	32
Laboratory practice	A2 A9 A11 A13 A26 A30 A31 B6	40	40	80
Seminar	A26 A27 B1 B2 B4 B5 B7 B10 B11 C3	4	8	12
Case study	B2 B5 B8	4	16	20
Mixed objective/subjective test	B3 B8 C1	2	0	2

Personalized attention		4	0	4

(*)The information in the planning table is for guidance only and does not take into account the heterogeneity of the students.

Methodologies	Description
Guest lecture / keynote speech	Exposition by teachers in which the theoretical program of the subject will be developed.
Laboratory practice	Students will conduct mandatory laboratory practices, which will be in group. The student will be introduced in the use of different techniques of analysis and study of microorganisms. In addition, microbiological analysis for different practical cases will be proposed and scientific criticism should be exercised.
Seminar	Works in small groups in which the results previously obtained in the laboratory practices will be presented in a reasoned manner.
Case study	The student will be proposed with at least one practical case in which he will reasonably indicate the actions to be taken from the point of view of a microbiologist, to meet the demand required in this case.
Mixed objective/subjective test	Test written in which the degree of knowledge and understanding achieved by the students in all aspects included in the subject will be assessed.

Methodologies	Competencies	Description	Qualification
Seminar	A26 A27 B1 B2 B4 B5 B7 B10 B11 C3	Evaluation of the tasks carried out during the seminars. It will be required by the students, in person and in the classroom, the results that have been obtained in the performing of laboratory practices.	10
Guest lecture / keynote speech	A1 A14 A15 A21 A25 A29 B12 C6 C8	Assessed through the mixed test.	0
Laboratory practice	A2 A9 A11 A13 A26 A30 A31 B6	Mandatory attendance and evaluation of student work during the development of practices. In mixed test, questions directly related to practical issues will be also proposed.	15
Mixed objective/subjective test	B3 B8 C1	Test written about the knowledge acquired in the keynote sessions, the laboratory practices and in the seminars.	60
Case study	B2 B5 B8	The student must resolve a practical case that will be proposed.	15

Assessment comments
To pass the course, in any of the diets to which the student may go, the student must have obtained a 5 out of 10 in the different parts of the "mixed test", performing all tasks that are considered mandatory, and obtain a minimum score of 2 points on a maximum 4. To account for the final grade in the value obtained in sections of seminars, practical and case study, the student must have passed the mixed test, corresponding to the theory of the subject. In order to be evaluated, students must attend to practical sessions. In the case of not passing the subject in a first option, in the second option, the student must pass only the part that was not passed. For a student to be considered "NOT PRESENT", he must have the following requirements: not site the examination (the mixed-test) and not attend half of the practice sessions. If the number of "with Honours" that may be granted is exhausted in the first option, none will be granted in the second option, even though the maximum note is obtained.

Basic	Madigan, Martinko, Bender, Buckley y Stahl (2015). Brock. Biología de microorganismos. 14º ed.. Pearson Education WILEY, SHERWOOD & WOOLVERTON (2009). Microbiología de Prescott, Harley y Klein. 7ª ed.. McGraw Hill

Complementary	COLLINS, LYNE & GRANGE (1995). Collins and Lyne's Microbiological Methods. 7th ed.. Butterworth-Heinemann Ltd. SINGER (2001). Experiments in Applied Microbiology. Academic Press HUDSON & SHERWOOD (1997). Explorations in Microbiology. Prentice Hall GAMAZO, LÓPEZ-GOÑI & DÍAZ (2005). Manual Práctico de Microbiología. 3ª ed.. Editorial Masson APHA, AWWA, WPCF (1992). Métodos normalizados para el análisis de aguas potables y residuales. Ediciones Díaz de Santos, S.A. PASCUAL ANDERSON & CALDERON PASCUAL (2000). Microbiología alimentaria. Metodología Analítica para alimentos y bebidas. Ediciones Díaz de Santos S.A.

Other comments
All the PowerPoint presentations corresponding to the class lectures are a guide for the study of the topics and they don´t constitute the total content of the subject.