| Topic |
Sub-topic |
| 1.- GENOME ORGANIZATION |
C-value paradox. Prokaryotic and eukaryotic genomes. Single-copy and repetitive DNA sequences. Gene families. Centromeres. Telomeres. Organelle genomes. |
| 2.- DNA REPLICATION |
Semiconservative DNA replication: the Meselson and Stahl experiment. Modes of replication. Enzymology of the replication. DNA replication in Escherichia coli. DNA replication in eukaryotes. Telomere synthesis. Replication of mithocondrial and cloroplast DNA.
|
| 3.- SYNTHESIS AND PROCESSING OF RNA |
Clases of RNA. RNA polymerases. Promoters and transcriptional appartatus. Transcription in prokaryotes and eukaryotes: initiation, elongation and termination. Interrupted genes: exons and introns. Processing of eukaryotic pre-mRNA. Synthesis and processing of pre-rRNA. Synthesis and processing of pre-tRNA. RNA edition. Revision of gene concept.
|
| 4.- TRANSLATION |
The one gene-one enzyme hypothesis. The genetic code: characteristics and experiments to decipher the code. Initiation of translation. Elongation of the polypeptide chain. Termination of translation. Messenger RNA surveillance.
|
| 5.- MUTATION AND DNA REPAIR |
Molecular basis of spontaneous mutations: replication errors, unequal crossing over, spontaneous chemical changes. Molecular basis of induced mutations: chemical and physical agents. Repair mechanisms: direct reversal of damaged DNA, excision repair, postreplication repair, error-prone repair, repair of double-strand breaks.
|
6.- MOLECULAR MECHANISM OF GENETIC RECOMBINATION
|
The role of genetic recombination. Gene conversion. Models of homologous recombination: Holliday model and double-strand break model. Enzymes required for recombination. Site-specific recombination. Inmunoglobulin genes assemble by recombination.
|
| 7.- TRANSPOSABLE GENETIC ELEMENTS |
Transposable elements in prokaryotes: insertion sequences, composite transposons and noncomposite transposons. Replicative and non replicative transposition. Transposable elements in eukaryotes: transposons and retrotransposon. Evolutionary significance of transposable elements.
|
| 8.- RECOMBINANT DNA TECHNOLOGY |
Restriction enzymes. Cloning vectors. DNA libraries: construction and screening. Southern and northern blotting. Restriction maps. Sequencing. PCR. Site-directed mutagenesis.
|
9.- APPLICATIONS OF RECOMBINANT DNA TECHNOLOGY
|
Expression of eukaryotic genes in E. coli. DNA transfer to eukaryotic cells. Transgenic animals. Transgenic plants. Gene therapy. Molecular markers. DNA fingerprinting. Genetic diagnosis. Synthetic genomes.
|
10.- GENOMICS
|
Physical and genetic mapping. Whole genome sequencing. Genome annotation. DNA microarrays. Reverse Genetics. Comparative genomics. Metagenomics.
|
11.- REGULATION OF GENE EXPRESSION IN BACTERIA
|
Jacob and Monod’s operon model for the regulation of lac genes in E. coli. Positive control of the lac operon. The arabinose operon of E. coli: positive and negative control. The triptophan operon of E. coli: negative control and attenuation. Control by RNA molecules. |
12.- REGULATION OF GENE EXPRESSION IN EUKARYOTES
|
Changes in chromatin structure. DNA metilation. Transcriptional control.
RNA processing control. Control of mRNA stability. Control at the level of translation. RNA interference. Epigenetics.
|
13.- GENETIC CONTROL OF DEVELOPMENT
|
Basic events of development. Drosophila development stages. Maternal-effect, segmentation and homeotic genes in Drososphila. Homeobox genes in other organisms. General aspects of Caenorhabditis elegans development. Genetic control of flower development in Arabidopsis. |
| PRACTICE 1: DNA EXTRACTION |
DNA extraction from Drosophila melanogaster and human cells. Agarose gel electrophoresis of DNA. DNA quantification. |
| PRACTICE 2: PCR |
PCR amplification of the locus PV92. Analysis of an insertion polymorphism of Alu sequences |
| PRACTICE 3: DOT-BLOT |
Nucleic acids hybridization: detection of microsatellite sequences by dot-blot |
| PRACTICE 4: BIOINFORMATICS. |
Database search and comparison of nucleic acid sequences. Primer design. Identification of ORFs. |
Subjects