Teaching GuideTerm Faculty of Science |
Mestrado Universitario en Biotecnoloxía Avanzada |
Subjects |
Biotechnological tools for forensic analysis |
Contents |
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Identifying Data | 2021/22 | |||||||||||||
Subject | Biotechnological tools for forensic analysis | Code | 610475505 | |||||||||||
Study programme |
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Descriptors | Cycle | Period | Year | Type | Credits | |||||||||
Official Master's Degree | 2nd four-month period |
First | Optional | 3 | ||||||||||
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Topic | Sub-topic |
Lecture 1. Biological material in forensic investigation | 1.1.Collection, characterization and storage of the samples. 1.2. Evidence collection. 1.3. Storage of biological material. |
Lecture 2. DNA extractionand quantification in forenxic analysis. | 2.1. DNA extraction techniques: Chelex, FTATM, IQR DNA system, differential DNA extraction, solid phase DNA extraction. 2.2. The polymerase chain reaction: PCR inhibition, sensitivity and contamination, RT_PCR and PCR multiplex. |
Lecture 3. Microsatellite DNA Typing | 3.1. Structure of STR loci. Development of STRs multiplexes, Interpretation of STR profiles.Assessment of STR profiles: stutter peaks, split peaks, pull-up, overloaded profiles. 3.2.Mini-STRs in mass disasters, and low copy number DNA 3.3. DNA databases in forensic genetics: CODIS, NDNAD and other european databases. |
Lecture 4. The X and Y chromosomes in forensics | 4.1.The structure of the sex chromosomes. 4.2. Short tandem repeats on sex chromosomes: power of ChrX markers in trace analysis, kinship testing and haplotype analysis. 4.3. Identification of male lineages: male´s paternity, males´s geographical origin. 4.4. Ethical considerations in ChrX marker testing. |
Lecture 5. SNPs in forensic casework | 5.1. Structure and detection of SNPs. 5.2. SNP typing technology 5.3. SNPs for human identification 5.4. Ethical considerations of SNP genotyping |
Lecture 6. Mitochondrial analysis in forensic science | 6.1. Mitochondrial DNA biology. 6.2. Heteroplasmy: concept and interpretation 6.3. Identification of individuals (mtDNA typing) |
Lecture 7. Aplications of forensic genetics in anaimal and plant species. | 7.1. Identification of species 7.2. Trazability and comercial fraud. Illegal trade and killing of protected species 7.3. Sex determination in birds |
Lecture 8. Statistical analysis of forensic data | 8.1. Introduction 8.2. Basic statistics in forensic genetics 8.3. The Hardy-Weinberg equilibrium. 8.4. Statistical parameters in forensic genetics: patarnity testing, individual identification and criminalistic |
Laboratory, blackboard and computer practices | Practice 1. Differential extraction of DNA from different sources Practice 2. DNA cuantification and PCR amplication Practice 3. Statistical analysis of the data |
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