Teaching GuideTerm
Faculty of Science
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Grao en Nanociencia e Nanotecnoloxía
 Subjects
  Fundamentals of Biotechnology
   Contents
Topic Sub-topic
1. INTRODUCTION TO BIOTECHNOLOGY Current concept of Biotechnology. History and development of Biotechnology. Outlook. Importance of Nanobiotechnology.
2. CLONING Purposes of molecular cloning. Basic steps of gene cloning. Polymerase chain reaction. DNA fragmentation: restriction enzymes. Binding of DNA molecules. Cloning and Nanotechnology.
3. GENOMIC LIBRARIES Library concept. Genomic DNA libraries. cDNA libraries. Expression libraries. Amplification, storage and replication of libraries. Libraries in Nanotechnology.
4. PROTEIN ENGINEERING Production of heterologous proteins in bacteria and yeast (Selection of microorganisms. Expression vectors. Expression in transformed cells. Secretion.) Production of recombinant proteins in animal cells (Expression vectors. Baculovirus-mediated protein expression in insect cell cultures ). Applications in Nanotechnology.
5. PROTEIN INMOBILIZATION Enzyme stability. Immobilized biocatalyst concept. immobilization systems. Nano-scale immobilization.
6. TRANSFORMATION AND GENE EDITING Concepts of modification, transformation and gene editing. Direct and indirect genetic transformation methods. Transgenic plants and animals. Transgenic foods. Nanotechnology in genetic modification.
7. CELL AND TISSUE ENGINEERING Introduction to animal and plant cell cultures. Types of crops. Cell culture requirements. Quantification of cellular parameters. contaminations. Cytotoxicity.
8. ETHICAL AND LEGAL ASPECTS Current situation of regulatory regulations, patents. Ethical issues, security, risks. Social perception.
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