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Teaching GuideTerm Faculty of Science |
| Mestrado Universitario en Bioloxía Molecular , Celular e Xenética |
 Subjects |
| Molecular Techniques |
| Contents |
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| Identifying Data | 2019/20 | |||||||||||||
| Subject | Molecular Techniques | Code | 610441002 | |||||||||||
| Study programme |
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| Descriptors | Cycle | Period | Year | Type | Credits | |||||||||
| Official Master's Degree | 1st four-month period |
First | Obligatory | 6 | ||||||||||
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| Topic | Sub-topic |
| Purification of Biomolecules | Principle of centrifugation technique and instrumentation. Preparative and Analytical Centrifugation. Chromatographic Techniques: principle and selection criteria. Electrophoresis: principle and types. Isoelectric focusing technique. Capillary electrophoresis. |
| PCR | Advanced concepts in PCR Differences between PCR and Real-time PCR Detection methods of amplicons Trial design and results analysis |
| Tecnology of molecular markers | Molecular markers: definition and main characterisics Basic principles, development and genotyping of RFLPs, AFLPs, microsatellites and SNPs |
| Recombinant DNA | Enzymes and protocols used in recombinant DNA techniques Genomics GeneBank Expression GeneBank GeneBank analysis Transfer and Blotting techniques Sequencing techniques Site-direct mutagenesis techniques Silencing techniques Transgenic organisms: uses and applications |
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