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Teaching GuideTerm Faculty of Science |
| Grao en Bioloxía |
 Subjects |
| Genetics |
| Contents |
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| Identifying Data | 2023/24 | |||||||||||||
| Subject | Genetics | Code | 610G02019 | |||||||||||
| Study programme |
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| Descriptors | Cycle | Period | Year | Type | Credits | |||||||||
| Graduate | 2nd four-month period |
Second | Obligatory | 6 | ||||||||||
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| Topic | Sub-topic |
| 1. Introduction to Genetics | Subdisciplines of Genetics Model organisms History of Genetics |
| 2. Mendelian Genetics | Mendel’s experiments: mono and dihibrid crosses Concept of geno and phenotype Terms and symbols Pedigree analysis |
| 3. Chromosomal Basis of Inheritance and Sex Determination | Genetic implications of mitosis and meiosis Chromosomal theory of inheritance Sex determination Sex-linked inheritance Sex-limited and sex-influenced traits Gene dosage compensation |
| 4. Extensions of and Deviations from Mendelian Genetic Principles | Modification of dominance relationships Multiple alleles Lethality Penetrance and expressivity Pleiotropy Gene interaction and epistasis Position effect Environmental interactions |
| 5. Genetic Mapping in Eukaryotes | Linkage, recombination and mapping of genes on chromosomes Interference and coincidence Genetic map function |
| 6. Genetic Analysis and Mapping in Bacteria and Bacteriophages | Mapping bacterial genes using conjugation, transformation and trnasduction Genetic recombination and gene mapping in bacteriophages Fine structure of the gene: rII system of phage T4 |
| 7. Extranuclear Inheritance | Infectious inheritance Organelle inheritance Heteroplasmy |
| 8. Quantitative Genetics |
Types of quantitative traits Infinitesimal model Components of the phenotypic variance Heritability |
| 9. Population Genetics | Allele and genotype frequencies Hardy-Weinberg equilibrium and departures |
| 10. DNA Organization in Chromosomes |
Bacterial and viral genomes Genome size: the C-value paradox Eukaryote chromosomes DNA packaging: Nucleosomes and Chromatin Centromeres and Telomeres Lampbrush and polytene chromosomes Karyotype |
| 11. DNA Mutation |
Types of mutations Spontaneous and induced mutation |
| 12. Variations in Chromosome Structure |
Deletions Duplications Inversions Translocations |
| 13. Variations in Chromosome Number | Euploidy and aneuploidy Trisomic analysis Polyploidy: autopolyploidy, allopoliploidy |
| 14. Developmental Genetics | Maternal effect genes and zygotic genes controlling embryionic development in metazoans |
| Teaching labs | Lab 1. GENETIC ANALYSIS IN CORN (Zea mays): INTERACTION AND EPISTASIS Description of shape and colour of F2 seeds (kernel) obtained from different crosses Hypothesis testing (chi-square) Inference of genotype and phenotype of generations P and F1 Genetic and Biochemistry basis of the observed phenotypes Lab 2. SETTING UP EXPERIMENTS USING Drosophila sp. Raising and handling Drosophila in the lab Life cycle Analysing fruit flies: distinguishing sex and observation of some mutant phenotypes Lab 3. LINKAGE MAPPING IN Drosophila sp. Reciprocal crosses between wild an mutant strains Analysis of offspring (F1) Testcrosses, analysis of offspring (F2) and statistical approach to determine the linkage order and map distances Lab 4. POLYTENE CHROMOSOME OF THE SALIVARY GLANDS OF Drosophila sp. Extraction of larval salivary glands Staining with orcein Examination of the slides under the microscope Lab 5. COMPUTER LAB. Introduction to bioinformatics databases and resources offered through the NCBI Getting familiar with the following databases: PUBMED, BOOKS, TAXONOMY, OMIM. |
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