Teaching GuideTerm
Faculty of Science
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Mestrado Universitario en Biotecnoloxía Avanzada
  Biotechnological tools for forensic analysis
Topic Sub-topic
Lecture 1. Biological material in forensic investigation 1.1.Collection, characterization and storage of the samples.
1.2. Evidence collection.
1.3. Storage of biological material.
Lecture 2. DNA extractionand quantification in forenxic analysis. 2.1. DNA extraction techniques: Chelex, FTATM, IQR DNA system, differential DNA extraction, solid phase DNA extraction.
2.2. The polymerase chain reaction: PCR inhibition, sensitivity and contamination, RT_PCR and PCR multiplex.
Lecture 3. Microsatellite DNA Typing 3.1. Structure of STR loci. Development of STRs multiplexes, Interpretation of STR profiles.Assessment of STR profiles: stutter peaks, split peaks, pull-up, overloaded profiles.
3.2.Mini-STRs in mass disasters, and low copy number DNA
3.3. DNA databases in forensic genetics: CODIS, NDNAD and other european databases.
Lecture 4. The X and Y chromosomes in forensics 4.1.The structure of the sex chromosomes.
4.2. Short tandem repeats on sex chromosomes: power of ChrX markers in trace analysis, kinship testing and haplotype analysis.
4.3. Identification of male lineages: male´s paternity, males´s geographical origin.
4.4. Ethical considerations in ChrX marker testing.
Lecture 5. SNPs in forensic casework 5.1. Structure and detection of SNPs.
5.2. SNP typing technology
5.3. SNPs for human identification
5.4. Ethical considerations of SNP genotyping
Lecture 6. Mitochondrial analysis in forensic science 6.1. Mitochondrial DNA biology.
6.2. Heteroplasmy: concept and interpretation
6.3. Identification of individuals (mtDNA typing)
Lecture 7. Aplications of forensic genetics in anaimal and plant species. 7.1. Identification of species
7.2. Trazability and comercial fraud. Illegal trade and killing of protected species
7.3. Sex determination in birds
Lecture 8. Statistical analysis of forensic data 8.1. Introduction
8.2. Basic statistics in forensic genetics
8.3. The Hardy-Weinberg equilibrium.
8.4. Statistical parameters in forensic genetics: patarnity testing, individual identification and criminalistic
Laboratory, blackboard and computer practices Practice 1. Differential extraction of DNA from different sources

Practice 2. DNA cuantification and PCR amplication

Practice 3. Statistical analysis of the data
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